Development and validation of KASP markers for resistance to Phytophthora capsici in Capsicum annuum L

文献类型： 外文期刊

第一作者： Zhang, Zhenghai

作者： Zhang, Zhenghai;Cao, Yacong;Wang, Yongfu;Yu, Hailong;Wu, Huamao;Liu, Jing;An, Dongliang;Zhu, Yanshu;Feng, Xigang;Zhang, Baoxi;Wang, Lihao

作者机构：

关键词： Capsicum annuum; KASP markers; GWAS; Phytophthora capsici

期刊名称：MOLECULAR BREEDING （ 影响因子：3.1； 五年影响因子：3.1 ）

ISSN： 1380-3743

年卷期： 2023 年 43 卷 3 期

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收录情况： SCI

摘要： Resistance of Capsicum annuum to Phytophthora blight is dependent on the genetic background of the resistance source and the Phytophthora capsici isolate, which poses challenges for development of generally applicable molecular markers for marker-assisted selection. In this study, the resistance to P. capsici of C. annuum was genetically mapped to chromosome 5 within a 1.68-Mb interval by genome-wide association study analysis of 237 accessions. In this candidate region, 30 KASP markers were developed using genome resequencing data for a P. capsici-resistant line (0601 M) and a susceptible line (77,013). Seven of these KASP markers, located in the coding region of a probable leucine-rich repeats receptor-like serine/threonine-protein kinase gene (Capana05g000704), were validated in the 237 accessions, which showed an average accuracy of 82.7%. The genotyping of the seven KASP markers strongly corresponded with the phenotype of 42 individual plants in a pedigree family (PC83-163) developed from the P. capsici-resistant line CM334. This research provides a set of efficient and high-throughput KASP markers for marker-assisted selection of resistance to P. capsici in C. annuum.

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