Riemerella anatipestifer UvrC is required for iron utilization, biofilm formation and virulence

文献类型： 外文期刊

第一作者： Wang, Jialing

作者： Wang, Jialing;Zou, Zuocheng;Hu, Mengmeng;Shan, Xinggen;Zhang, Ying;Miao, Yiqin;Zhang, XiaoYing;Islam, Nazrul;Hu, Qinghai

作者机构：

关键词： Riemerella anatipestifer; UvrC; biofilm formation; virulence; iron utilization; oxidative stress tolerance

期刊名称：AVIAN PATHOLOGY （ 影响因子：2.8； 五年影响因子：3.2 ）

ISSN： 0307-9457

年卷期： 2024 年

页码：

收录情况： SCI

摘要： UvrC is a subunit of excinuclease ABC, which mediates nucleotide excision repair (NER) in bacteria. Our previous studies showed that transposon Tn4531 insertion in the UvrC encoding gene Riean_1413 results in reduced biofilm formation by Riemerella anatipestifer strain CH3 and attenuates virulence of strain YZb1. In this study, whether R. anatipestifer UvrC has some biological functions other than NER was investigated. Firstly, the uvrC of R. anatipestifer strain Yb-2 was in-frame deleted by homologous recombination, generating deletion mutant Delta uvrC, and its complemented strain c Delta uvrC was constructed based on Escherichia coli - R. anatipestifer shuttle plasmid pRES. Compared to the wild-type (WT) R. anatipestifer strain Yb2, uvrC deleted mutant Delta uvrC significantly reduced biofilm formation, tolerance to H2O2- and HOCl-induced oxidative stress, iron utilization, and adhesion to and invasion of duck embryonic hepatocytes, but not its growth curve and proteolytic activity. In addition, animal experiments showed that the LD50 value of Delta uvrC in ducklings was about 13-fold higher than that of the WT, and the bacterial loads in Delta uvrC infected ducklings were significantly lower than those in Yb2-infected ducklings, indicating uvrC deletion in R. anatipestifer attenuated virulence. Taken together, the results of this study indicate that R. anatipestifer UvrC is required for iron utilization, biofilm formation, oxidative stress tolerance and virulence of strain Yb2, demonstrating multiple functions of UvrC.

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