Development of a competitive ELISA using monoclonal antibodies for detecting neutralizing antibodies against Peste-des-petits-ruminants virus
文献类型： 外文期刊
第一作者： Hu, Linjie
作者： Hu, Linjie;Xu, Haoyue;Zhu, Xueliang;Peng, Hongjiao;Deng, Ruixue;Sun, Yuefeng;Meng, Xuelian
作者机构：
关键词： Peste-des-petits-ruminants virus; Haemagglutinin protein; Monoclonal antibody; Competitive ELISA
期刊名称：VETERINARY JOURNAL （ 影响因子：3.1； 五年影响因子：2.7 ）
ISSN： 1090-0233
年卷期： 2025 年 313 卷
页码：
收录情况： SCI
摘要： Peste-des-petits-ruminants (PPR) is an acute, contact infectious disease caused by peste-des-petits-ruminants virus (PPRV) infection. PPRV mainly infects goats, sheep and other small ruminants, posing a serious threat to the development of the livestock industry and the safety of wildlife. In this study, a competitive ELISA (c-ELISA) method for detecting neutralizing antibodies against PPRV was developed using the extracellular domain of haemagglutinin (tH) protein and a monoclonal antibody against tH protein. The results showed that the specificity and sensitivity of c-ELISA were 99.38% and 100%, respectively. The agreement rate of the c-ELISA and viral neutralization test (VNT) was 96.65%, suggesting that the c-ELISA could detect neutralizing antibody against PPRV and could be used for protective immunity evaluation.
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