A simple, rapid and effective method for total RNA extraction from Lentinula edodes

文献类型： 外文期刊

第一作者： Li, Jun-hui

作者： Li, Jun-hui;Tang, Chuan-hong;Song, Chun-yan;Chen, Ming-jie;Feng, Zhi-yong;Pan, Ying-jie

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期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： A rapid, inexpensive and reliable method for total RNA extraction from fruiting bodies of Lentinula edodes containing large quantities of polysaccharides and secondary metabolites is described. An initial extraction step using saturated NaCl solution facilitates the separation of nucleic acids from contaminants and, after further extraction with organic solvents and precipitation with 2-propanol, total RNA of high purity and suitable for applications such as cDNA synthesis, RT-PCR and Northern blot hybridization was obtained. The procedure may also have wider applicability for total RNA extraction from the tissues of other mushrooms.

分类号： Q81`Q1-0`Q7

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