Influence of thermophilic microorganism on non-volatile metabolites during high-temperature pile-fermentation of Chinese dark tea based on metabolomic analysis

文献类型: 外文期刊

第一作者: Zhu, Wen

作者: Zhu, Wen;Wang, Wenfeng;Xu, Wencan;Wu, Shuang;Chen, Wenjun;Huang, Youyi;Zhu, Wen;Wang, Wenfeng;Xu, Wencan;Wu, Shuang;Chen, Wenjun;Huang, Youyi;Wang, Shengpeng

作者机构:

关键词: Thermophilic microorganism; High-temperature; Chinese dark tea; Pile-fermentation; Nontargeted metabolomic analysis

期刊名称:FOOD SCIENCE AND BIOTECHNOLOGY ( 影响因子:3.231; 五年影响因子:2.925 )

ISSN: 1226-7708

年卷期: 2022 年 31 卷 7 期

页码:

收录情况: SCI

摘要: Pile-fermentation is a critical procedure for producing Chinese dark tea, during which thermophilic microorganisms would play an irreplaceable role. However, there have been little researches on the influences of thermophilic microorganism pile-fermentation (TMPF) in high-temperature of Chinese dark tea. Thus, we conducted high-performance liquid chromatography and nontargeted metabolomic to analyze the non-volatile metabolites of TMPF. Our results discovered that the amounts of ( -)-epigallocatechin gallate, ( -)-epigallocatechin, ( -)-epicatechin gallate, and ( -)-epicatechin were decreased significantly (p < 0.05) after TMPF. By using nontargeted metabolomic analysis, a total of 1733 ion features were detected. KEGG pathway enrichment analysis showed that TMPF had a significant impact on caffeine metabolism. Also, theophylline, 3-methylxanthine, and 1,3,7-trimethyluric acid were increased significantly after TMPF, which suggested that demethylation and oxidation reaction might be the main pathways of caffeine metabolism. This study provides a better understanding of the mechanism of TMPF during high-temperature for Chinese dark tea and lays a foundation for further research.

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