One-pot three-enzyme synthesis of UDP-Glc, UDP-Gal, and their derivatives

文献类型： 外文期刊

第一作者： Zou, Yang

作者： Zou, Yang;Xue, Mengyang;Liu, Jun;Wang, Peng George;Chen, Min;Zou, Yang;Xue, Mengyang;Liu, Jun;Wang, Peng George;Chen, Min;Wang, Wenjun;Shen, Jie;Wang, Wenjun;Shen, Jie;Cai, Li;Chen, Leilei

作者机构：

关键词： One-pot reaction system; Three enzymes; UDP-Glc/Gal derivatives

期刊名称：CARBOHYDRATE RESEARCH （ 影响因子：2.104； 五年影响因子：2.192 ）

ISSN： 0008-6215

年卷期： 2013 年 373 卷

页码：

收录情况： SCI

摘要： A UTP-glucose-1-phosphate uridylyltransferase (SpGalU) and a galactokinase (SpGalK) were cloned from Streptococcus pneumoniae TIGR4 and were successfully used to synthesize UDP-galactose (UDP-Gal), UDPglucose (UDP-Glc), and their derivatives in an efficient one-pot reaction system. The reaction conditions for the one-pot multi-enzyme synthesis were optimized and nine UDP-Glc/Gal derivatives were synthesized. Using this system, six unnatural UDP-Gal derivatives, including UDP-2-deoxy-Galactose and UDP-GalN(3) which were not accepted by other approach, can be synthesized efficiently in a one pot fashion. More interestingly, this is the first time it has been reported that UDP-Glc can be synthesized in a simpler one-pot three-enzyme synthesis reaction system. (C) 2013 Elsevier Ltd. All rights reserved.

分类号： O62

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