Enterococcus faecium as a causative agent of death of Small-Tailed Han sheep farmed in Tongliao region of China: A case study

文献类型： 外文期刊

第一作者： Meng, Qinglei

作者： Meng, Qinglei;Chen, Yunjiao;Liu, Zhilin;Zhang, Xin;Jia, Zelin;Cui, Jiayu;Wang, Xueli;Jian, Ruizhen;Jia, Weijuan

作者机构：

关键词： Sheep; Enterococcus faecium; Isolation; Antibiotics; Resistance genes

期刊名称：JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY （ 影响因子：0.4； 五年影响因子：0.5 ）

ISSN： 1792-2720

年卷期： 2025 年 76 卷 2 期

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收录情况： SCI

摘要： Enterococcus faecium is a facultative anaerobic Gram-positive bacterium that exhibits high levels of tolerance ( (Enterococcus faecium can survive in harsh environments such as acidic, alkaline and high temperature environments). Some strains of Enterococcus faecium have been utilized as probiotics; however, certain strains of Enterococcus faecium are also considered pathogenic, with the potential to cause significant health concerns in humans and animals. Enterococcus faecium has demonstrated a notable capacity for acquiring resistant genes, thereby complicating its control and prevention. Enterococcus faecium disease is a significant infectious disease that affects the health of sheep. There is a paucity of reports on the subject of pathogenic Enterococcus faecium of sheep origin. In this study, we reported a strain of Enterococcus faecium isolated from a Small-Tailed Han sheep farm in Tongliao, Inner Mongolia Autonomous Region, China. We perform tests for isolation and culture of bacteria, 16S rDNA identification, antimicrobial susceptibility test, detection of resistant genes, animal regression tests and observation of pathological sections. Tests confirm that the pathogen causing sheep deaths is Enterococcus faecium (GenBank sequence number: OP430848.1). Better growth of isolated strain on sheep blood agar medium was observed. The isolated strain exhibited 99.90% similarity to the 16S rDNA fragment of Enterococcus faecium that has been published on GenBank. The antimicrobial susceptibility test revealed that florfenicol and amikacin exhibited the optimal bacteriostatic effect. Isolated strain containing cfr, tetM and ant(6)-I resistance genes. The results of animal regression tests demonstrated the high pathogenicity of the isolated strain. The histopathologic results indicated the presence of pathological changes in the substantial organs (heart, liver, spleen and kidney) of the deceased sheep. This study provides a reference point for the diagnosis, treatment, and investigation of drug resistance in pathogenic Enterococcus faecium of sheep origin. The future rational use of antibiotics in animal husbandry to curb the spread of resistant Enterococcus faecium is of great importance.

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