Arabidopsis F-BOX STRESS INDUCED 4 is required to repress excessive divisions in stomatal development

文献类型： 外文期刊

第一作者： Li, Yi

作者： Li, Yi;Xue, Shan;He, Qixiumei;Wang, Junxue;Zhu, Lingling;Zou, Junjie;Zhang, Jie;Zuo, Chaoran;Fan, Zhibin;Yue, Junling;Zhang, Chunxia;Yang, Kezhen;Le, Jie;Li, Yi;He, Qixiumei;Zhu, Lingling;Zhang, Jie;Zuo, Chaoran;Fan, Zhibin;Yue, Junling;Le, Jie;Xue, Shan;Wang, Junxue;Zou, Junjie

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关键词： cell division; cell cycle; F-BOX; stomatal development; ubiquitination

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.106； 五年影响因子：8.241 ）

ISSN： 1672-9072

年卷期： 2022 年 64 卷 1 期

页码：

收录情况： SCI

摘要： During the terminal stage of stomatal development, the R2R3-MYB transcription factors FOUR LIPS (FLP/MYB124) and MYB88 limit guard mother cell division by repressing the transcript levels of multiple cell-cycle genes. In Arabidopsis thaliana possessing the weak allele flp-1, an extra guard mother cell division results in two stomata having direct contact. Here, we identified an ethylmethane sulfonate-mutagenized mutant, flp-1 xs01c, which exhibited more severe defects than flp-1 alone, producing giant tumor-like cell clusters. XS01C, encoding F-BOX STRESS-INDUCED 4 (FBS4), is preferentially expressed in epidermal stomatal precursor cells. Overexpressing FBS4 rescued the defective stomatal phenotypes of flp-1 xs01c and flp-1 mutants. The deletion or substitution of a conserved residue (Proline166) within the F-box domain of FBS4 abolished or reduced, respectively, its interaction with Arabidopsis Skp1-Like1 (ASK1), the core subunit of the Skp1/Cullin/F-box E3 ubiquitin ligase complex. Furthermore, the FBS4 protein physically interacted with CYCA2;3 and induced its degradation through the ubiquitin-26S proteasome pathway. Thus, in addition to the known transcriptional pathway, the terminal symmetric division in stomatal development is ensured at the post-translational level, such as through the ubiquitination of target proteins recognized by the stomatal lineage F-box protein FBS4.

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