Development of a Real-Time PCR Method for Identifying Four Major Freshwater Fish in Marine Fish Surimi

文献类型： 外文期刊

第一作者： Li, Yutong

作者： Li, Yutong;Li, Yutong;Qu, Meng;Liu, Enchong;Jiang, Yanhua;Guo, Yingying;Zhu, Wenjia;Li, Na;Wang, Lianzhu;Yao, Lin;Liu, Enchong;Wan, Shuo

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关键词： Marine fish surimi; Freshwater fish; Identification; ND5 gene; QPCR

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.0； 五年影响因子：3.1 ）

ISSN： 1936-9751

年卷期： 2025 年 18 卷 10 期

页码：

收录情况： SCI

摘要： BackgroundThe adulteration of marine fish surimi with freshwater fish without consumer disclosure is a growing concern in China. Thus, a rapid detection method is needed for four major freshwater species: black carp (Mylopharyngodon piceus), grass carp (Ctenopharyngodon idella), silver carp (Hypophthalmichthys molitrix), and bighead carp (Hypophthalmichthys nobilis).Methods and ResultsMitochondrial NADH dehydrogenase subunit 5 (ND5) sequences were aligned and analyzed to design species-specific primers and probes for a real-time quantitative polymerase chain reaction (qPCR) assay. This method enabled the simultaneous identification of M. piceus, C. idella, H. molitrix, and H. nobilis in marine fish surimi. The designed primers and probes demonstrated high specificity, with no cross-reactivity to 24 other fish species in the qPCR reaction. The detection limits of the method were 0.0005 ng mu L-1 for M. piceus and H. nobilis and 0.005 ng mu L-1 for C. idella and H. molitrix. The method detected M. piceus at 0.1%, C. idella at 0.01%, and both H. molitrix and H. nobilis at 0.001% in fish mixtures. Among 50 commercial samples, 31 tested positive for one or more of these species.ConclusionThe developed qPCR method specifically detects M. piceus, C. idella, H. molitrix, and H. nobilis in marine fish surimi and has potential for use in routine quality control by food regulators.

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