A novel glucan from Sparassis latifolia: purification, structural characterization and protective effect on gastric mucosal cells against ethanol-induced damage

文献类型： 外文期刊

第一作者： Wang, Mengyu

作者： Wang, Mengyu;Lv, Guoying;Zhao, Ruimeng;Cai, Weiming;Zhang, Zuofa

作者机构：

关键词： Sparassis latifolia; Polysaccharide; Structural characterization; Gastroprotective effect; Gastric mucosal cells; Underlying mechanisms

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：5.2； 五年影响因子：6.1 ）

ISSN：

年卷期： 2025 年 12 卷 1 期

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收录情况： SCI

摘要： Sparassis latifolia is a rare edible mushroom with many pharmacological activities. Active screening found that the crude polysaccharide from this mushroom (SLP) exhibited noticeable gastroprotective effects. However, it remains unclear regarding the underlying regulatory mechanism of the gastroprotective effects of SLP. A novel polysaccharide SLP-2 was purified from the fruiting bodies of S. latifolia, and characterized using FT-IR, HPAEC, SEM, SEC-MALLS-RI, methylation, and NMR analysis. Structural characterization revealed that the backbone of SLP-2 was mainly composed of -> 4)-alpha-D-Glcp-(1 -> , -> 3)-alpha-D-Glcp-(1 -> and -> 3,4)-alpha-D-Glcp-(1 -> . The branched chain primarily consisted of beta-D-Glcp-(1 -> linked to the sugar residue -> 3, 6)-beta-D-Glcp-(1 -> at the O-6 position and -> 3,4)-beta-D-Glcp-(1 -> at the O-4 position. Physiological experimental results demonstrated that SLP-2 effectively protected GES-1 cells against ethanol-induced damage by suppressing intracellular ROS levels and cell apoptosis while elevating the activities of SOD and CAT enzymes. Furthermore, transcriptome analysis revealed molecular mechanisms underlying the gastroprotective effect of SLP-2, with KEGG enrichment suggesting associations with ferroptosis, TNF, PI3K-Akt, MAPK and IL-17 signaling pathways. These findings provide a technological foundation for valuable utilization of S. latifolia and emphasize the potential application of SLP-2 in repairing gastric mucosal injuries.

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