Selenium Treatment Ameliorates the Adverse Effects Caused by Dynamin Gene Knockdown in Bombyx mori
文献类型： 外文期刊
第一作者： Hong, Ting-Ting
作者： Hong, Ting-Ting;Hu, Fei;Ge, Wen-Jie;Thakur, Kiran;Wei, Zhao-Jun;Hu, Shuo;Hu, Fei;Thakur, Kiran;Wei, Zhao-Jun;Tang, Shun-Ming;Tang, Shun-Ming
作者机构：
关键词： Bombyx mori; dynamin; gene expression; Se; siRNAs
期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.9； 五年影响因子：2.2 ）
ISSN： 0739-4462
年卷期： 2024 年 117 卷 1 期
页码：
收录情况： SCI
摘要：
Our previous research reported the influence of 50 mu M selenium (Se) on the cytosolization (endocytosis) pathway, which in turn stimulates the growth and development of Bombyx mori. Lately, dynamin is recognized as one of the key proteins in endocytosis. To explore the underlying mechanisms of Se impact, the dynamin gene was knocked down by injecting siRNAs (Dynamin-1, Dynamin-2, and Dynamin-3). This was followed by an analysis of the target gene and levels of silk protein genes, as well as growth and developmental indices, Se-enrichment capacity, degree of oxidative damage, and antioxidant capacity of B. mori. Our findings showed a considerable decrease in the relative expression of the dynamin gene in all tissues 24 h after the interference and a dramatic decrease in the silkworm body after 48 h. RNAi dynamin gene decreased the silkworm body weight, cocoon shell weight, and the ratio of cocoon. In the meantime, malondialdehyde level increased and glutathione level and superoxide dismutase/catalase activities decreased. 50 mu M Se markedly ameliorated these growth and physiological deficits as well as decreases in dynamin gene expression. On the other hand, there were no significant effects on fertility (including produced eggs and laid eggs) between the interference and Se treatments. Additionally, the Se content in the B. mori increased after the dynamin gene interference. The dynamin gene was highly expressed in the silk gland and declined significantly after interference. Among the three siRNAs (Dynamin-1, Dynamin-2, and Dynamin-3), the dynamin-2 displayed the highest interference effects to target gene expression. Our results demonstrated that 50 mu M Se was effective to prevent any adverse effects caused by dynamin knockdown in silkworms. This provides practical implications for B. mori breeding industry.
分类号：
- 相关文献
作者其他论文 更多>>
-
The Potential of Using Different Legume Species in a Push-Pull Approach to Manage Spodoptera frugiperda in Maize in China
作者：Bi, Sijia;Wang, Yinhao;Xu, Tingting;Hu, Benjin;Hu, Fei;Xu, Lina;Wang, Yinhao;Wang, Zhenying;Jatoi, Wajid Ali
关键词：cowpea; fall armyworm; mung bean; push-pull strategy; repellent and attractant plants
-
Inclusive analysis of key precursor peptides and flavor enhancing components of sesame cake meal maillard reaction products
作者：Lou, Peng-Xiang;Zhang, Jian-Guo;Zhang, Wang-Wei;Thakur, Kiran;Wei, Zhao-Jun;Thakur, Kiran;Wei, Zhao-Jun;Zhang, Yinping
关键词：Sesame seed cake meal; Cysteine; Maillard reaction products; Peptide; LC-MS/MS; Flavor
-
Recent updates on plant-derived anti-fatigue peptides as novel energy boosters: expansion from mechanisms of action and beyond
作者：Liao, Ai-Mei;Xu, Tingting;Pan, Long;Huang, Jihong;Liu, Na;Hu, Yuansen;Liao, Ai-Mei;Thakur, Kiran;Wei, Zhao-Jun;Hou, Yin-Chen;Huang, Jihong;Zhang, Kangyi;Liu, Xian-ming
关键词：Plant-derived oligopeptides; Anti-fatigue activity; Evaluation methods; Mechanism of anti-fatigue; Anti-fatigue signaling pathway
-
Immunotranscriptomic Profiling of Spodoptera frugiperda Challenged by Different Pathogenic Microorganisms
作者：Tang, Yan;Yu, Guojie;Zou, Qi;Liu, Feng;Wu, Yu;Zhao, Xueyan;Wang, Wensheng;Liu, Xinchang;Wang, Zengxia;Hu, Fei;Wang, Zengxia
关键词：
Spodoptera frugiperda ; pathogenic microorganisms; transcriptome; induced expression; immune genes -
Molecular Insights into Diapause Mechanisms in Telenomus remus for Improved Biological Control
作者：Yu, Guojie;Sheng, Longyu;Zhang, Zhongyue;Zou, Qi;Lai, Xinxin;Tang, Yan;Li, Yuyao;Liu, Jia;Yan, Hao;Xie, Xianglin;Hu, Fei;Wang, Zengxia;Wang, Zengxia
关键词：diapause;
Telenomus remus ;Spodoptera frugiperda ; transcriptome analysis; photoperiod; calcium signaling; pest control; sustainable agriculture -
MiRNA Omics Reveal the Mechanisms of the Dual Effects of Selenium Supplementation on the Development of the Silkworm (Bombyx mori)
作者：Ge, Wen-Jie;Hu, Fei;Hong, Ting-Ting;Thakur, Kiran;Zhang, Jian-Guo;Wei, Zhao-Jun;Hu, Fei;Thakur, Kiran;Wei, Zhao-Jun;Tang, Shun-Ming;Tang, Shun-Ming
关键词：Bombyx mori; miRNA; Se; gene regulation networks
-
Risk Assessment and Fitness Cost of Tetraniliprole Resistance in Ostrinia furnacalis (Lepidoptera: Crambidae)
作者：Xu, Tingting;Hu, Fei;Yue, Ran;Hu, Benjin;Bi, Sijia;Tong, Youmin;Xu, Lina;Xu, Tingting;Hu, Fei;Hu, Benjin;Bi, Sijia;Tong, Youmin;Xu, Lina;Yue, Ran
关键词：
Ostrinia furnacalis ; tetraniliprole; risk assessment; fitness cost
