Application of the rapid leaf A-C-i response (RACiR) technique: examples from evergreen broadleaved species

文献类型： 外文期刊

第一作者： Lin, Quanhong

作者： Lin, Quanhong;Tian, Di;Zhao, Changti;Lin, Quanhong;Tian, Di;Stocker, Benjamin D.;Tian, Di;Stocker, Benjamin D.;Wang, Bin;Li, Yu'e;Yan, Zhengbing;Fang, Jingyun;Fang, Jingyun

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关键词： Rapid A-C-i response (RACiR); Steady-state A-C-i; CO2 response; V-cmax; J(max); RuBisCO; Photosynthesis

期刊名称：PHOTOSYNTHESIS RESEARCH （ 影响因子：3.429； 五年影响因子：3.387 ）

ISSN： 0166-8595

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收录情况： SCI

摘要： Using steady-state photosynthesis-intercellular CO2 concentration (A-C-i) response curves to obtain the maximum rates of ribulose-1,5-bisphosphate carboxylase oxygenase carboxylation (V-cmax) and electron transport (J(max)) is time-consuming and labour-intensive. Instead, the rapid A-C-i response (RACiR) technique provides a potential, high-efficiency method. However, efficient parameter settings of RACiR technique for evergreen broadleaved species remain unclear. Here, we used Li-COR LI-6800 to obtain the optimum parameter settings of RACiR curves for evergreen broadleaved trees and shrubs. We set 11 groups of CO2 gradients ([CO2]), i.e. R1 (400-1500 ppm), R2 (400-200-800 ppm), R3 (420-20-620 ppm), R4 (420-20-820 ppm), R5 (420-20-1020 ppm), R6 (420-20-1220 ppm), R7 (420-20-1520 ppm), R8 (420-20-1820 ppm), R9 (450-50-650 ppm), R10 (650-50 ppm) and R11 (650-50-650 ppm), and then compared the differences between steady-state A-C-i and RACiR curves. We found that V-cmax and J(max) calculated by steady-state A-C-i and RACiR curves overall showed no significant differences across 11 [CO2] gradients (P > 0.05). For the studied evergreens, the efficiency and accuracy of R2, R3, R4, R9 and R10 were higher than the others. Hence, we recommend that the [CO2] gradients of R2, R3, R4, R9 and R10 could be applied preferentially for measurements when using the RACiR technique to obtain V-cmax and J(max) of evergreen broadleaved species.

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