Multi-omics-based study on metabolic-physiological response to heat stress in rainbow trout (Oncorhynchus mykiss)

文献类型： 外文期刊

第一作者： Yang, Yang

作者： Yang, Yang;Wu, Song;Sun, Yanchun;Han, Shicheng;Liu, Yingjie;Liu, Wenzhi;Han, Lin;Yuan, Fangying;Wang, Jing

作者机构：

关键词： Rainbow trout; Heat stress; Multi-omics; Adaptive strategies; Inflammation response

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY （ 影响因子：2.2； 五年影响因子：2.4 ）

ISSN： 1095-6433

年卷期： 2025 年 308 卷

页码：

收录情况： SCI

摘要： Global warming poses a critical survival challenge for cold-water economic fish, necessitating elucidation of their physiological and metabolic responses to heat stress. This study established a rainbow trout heat stress model using a gradual temperature increase protocol (from 16 degrees C to 20 degrees C over 8 days, followed by maintenance at 20 degrees C for 22 days) and investigated the adverse effects and adaptive strategies of rainbow trout under heat stress. Extensive congestion and steatosis observed in liver tissues suggested that heat stress induced inflammatory responses and dysregulation of lipid metabolism. Significant increases in antioxidant enzyme activities and reactive oxygen species levels (1.29-fold) indicated activation of the antioxidant defense system, and this response was insufficient to alleviate oxidative damage. Integrative transcriptomic and metabolomic analyses revealed that lipid metabolism was the most significantly altered biological process. Elevated prostaglandin D2 (1.64-fold change), driven by disruption of arachidonic acid metabolism, triggered inflammatory responses. Adaptive upregulation of linoleic acid and alpha-linolenic acid metabolism likely maintained membrane fluidity, enhancing the capacity for heat stress adaptation. Concurrently, adaptive activation of both GPX4A (6.73-fold change) and the heat shock proteins (LOC118938277 and hsp47) likely mitigated oxidative damage associated with heat stress in the liver. Overall, this study advances the understanding of pathological injuries and adaptive strategies in rainbow trout under heat stress and identifies candidate genes for breeding heat-tolerant strains.

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