The divergence of DHN-derived melanin pathways in Metarhizium robertsii

文献类型： 外文期刊

第一作者： Xie, Linan

作者： Xie, Linan;Liu, Yang;Chen, Kang;Yue, Qun;Wang, Chen;Xu, Yuquan;Zhang, Liwen;Zhang, Yujie;Dun, Baoqing

作者机构：

关键词： Dihydroxynaphthalene-(DHN) melanin; Entomopathogenic fungi; Metarhizium spp.; Polyketide synthase (PKS); Stress resistance

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN： 0959-3993

年卷期： 2024 年 40 卷 10 期

页码：

收录情况： SCI

摘要： The important role of dihydroxynaphthalene-(DHN) melanin in enhancing fungal stress resistance and its importance in fungal development and pathogenicity are well-established. This melanin also aids biocontrol fungi in surviving in the environment and effectively infecting insects. However, the biosynthetic origin of melanin in the biocontrol agents, Metarhizium spp., has remained elusive due to the complexity resulting from the divergence of two DHN-like biosynthetic pathways. Through the heterologous expression of biosynthetic enzymes from these two pathways in baker's yeast Saccharomyces cerevisiae, we have confirmed the presence of DHN biosynthesis in M. roberstii, and discovered a novel naphthopyrone intermediate, 8, that can produce a different type of pigment. These two pigment biosynthetic pathways differ in terms of polyketide intermediate structures and subsequent modification steps. Stress resistance studies using recombinant yeast cells have demonstrated that both DHN and its intermediates confer resistance against UV light prior to polymerization; a similar result was observed for its naphthopyrone counterpart. This study contributes to the understanding of the intricate and diverse biosynthetic mechanisms of fungal melanin and has the potential to enhance the application efficiency of biocontrol fungi such as Metarhizium spp. in agriculture.

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