Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet
文献类型： 外文期刊
第一作者： Liu, Yang
作者： Liu, Yang;Chen, Weiyao;Chen, Jinfeng;Cheng, Zixiang;Wu, Chuanyin;Sui, Yi;Chen, Weiyao
作者机构： Chinese Acad Sci, Inst Zool, State Key Lab Integrated Management Pest Insects &, Beijing 100101, Peoples R China;Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China;Univ Chinese Acad Sci, Beijing 100049, Peoples R China
关键词： broomcorn millet; CRISPR/Cas9 editing; genome assembly; herbicide resistance; transformation system
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 2022影响因子：11.4； 五年影响因子：10.1 ）
ISSN： 1672-9072
年卷期： 2024 年
收录情况： SCI
摘要： The ancient crop broomcorn millet (Panicum miliaceum L.) is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance. These advantages make it an important alternative crop to increase food security and achieve the goal of zero hunger, particularly in light of the uncertainty of global climate change. However, functional genomic and biotechnological research in broomcorn millet has been hampered due to a lack of genetic tools such as transformation and genome-editing techniques. Here, we successfully performed genome editing of broomcorn millet. We identified an elite variety, Hongmi, that produces embryogenic callus and has high shoot regeneration ability in in vitro culture. We established an Agrobacterium tumefaciens-mediated genetic transformation protocol and a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-mediated genome-editing system for Hongmi. Using these techniques, we produced herbicide-resistant transgenic plants and edited phytoene desaturase (PmPDS), which is involved in chlorophyll biosynthesis. To facilitate the rapid adoption of Hongmi as a model line for broomcorn millet research, we assembled a near-complete genome sequence of Hongmi and comprehensively annotated its genome. Together, our results open the door to improving broomcorn millet using biotechnology.
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