Identification and subcellular localization analysis of CCT alpha in microsporidian Nosema bombycis

文献类型： 外文期刊

第一作者： Yao, Mingshuai

作者： Yao, Mingshuai;Wang, Runpeng;Chen, Ying;He, Ping;Wei, Erjun;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan;Yao, Mingshuai;Zhu, Feng;Wang, Qiang;Zhang, Yiling;Tang, Xudong;Shen, Zhongyuan

作者机构：

关键词： Nosema bombycis; Chaperonin; NbCCT alpha; Subcellular localization; RNAi

期刊名称：INFECTION GENETICS AND EVOLUTION （ 影响因子：4.393； 五年影响因子：3.656 ）

ISSN： 1567-1348

年卷期： 2022 年 102 卷

页码：

收录情况： SCI

摘要： CCT is a chaperonin which is widely present in eukaryotic cells and mainly involves in the folding and assembly of cytoskeletal proteins beta-tubulin and actin. The alpha subunit of CCT(CCT alpha) plays a pivotal role in the folding and assembly of cytoskeletal protein(s) as an individuals or complexes. In this study, we report cloning, characterization and expression of the CCT alpha of Nosema bombycis (NbCCT alpha) for the first time. The NbCCT alpha gene contains a complete ORF of 1629 bp in length that encodes a 542-amino acid polypeptide. The NbCCT alpha is 59.662 kDa molecular weight in size with an isoelectric point (pI) of 5.81, no signal peptide or transmembrane domain. The IFA results showed that the NbCCT alpha was co-localized with actin and beta-tubulin in the cytoplasm, nucleus, nuclear membrane and plasma membrane of N. bombycis in the process of proliferation. qPCR analysis showed that the relative expression level of NbCCT alpha increased from 24 h to 96 h post-infection (hp.i) of N. bombycis, and reached the highest at 96 hp.i. The relative expression level of NbCCT alpha gene after RNAi was restrained at a low level from 48 hp.i to 96 hp.i. Knockdown of NbCCT alpha gene down-regulated the expression of Nb beta-tubulin and Nbactin genes. These results imply that NbCCT alpha may play an important role in the lifecycle of N. bombycis.

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