Molecular characterization and functional analysis of Eimeria tenella ankyrin repeat-containing protein

文献类型： 外文期刊

第一作者： Guo, Huilin

作者： Guo, Huilin;Zhao, Qiping;Wang, Haixia;Zhu, Shunhai;Dong, Hui;Xie, Xinrui;Wang, Lihui;Chen, Lang;Han, Hongyu

作者机构：

关键词： Eimeria tenella; Ankyrin repeat-containing protein; Invasion; Drug resistance

期刊名称：EUROPEAN JOURNAL OF PROTISTOLOGY （ 影响因子：2.9； 五年影响因子：2.4 ）

ISSN： 0932-4739

年卷期： 2024 年 94 卷

页码：

收录情况： SCI

摘要： Chicken coccidiosis causes disastrous losses to the poultry industry all over the world. Eimeria tenella is the most prevalent of these disease-causing species. Our former RNA-seq indicated that E. tenella ankyrin repeat-containing protein (EtANK) was expressed differently between drug-sensitive (DS) and drug-resistant strains. In this study, we cloned EtANK and analyzed its translational and transcriptional levels using quantitative real-time PCR (qPCR) and western blotting. The data showed that EtANK was significantly upregulated in diclazuril-resistant (DZR) strain and maduramicin-resistant (MRR) strain compared with the drug-sensitive (DS) strain. In addition, the transcription levels in the DZR strains isolated from the field were higher than in the DS strain. The translation levels of EtANK were higher in unsporulated oocysts (UO) than in sporozoites (SZ), sporulated oocysts (SO), or second-generation merozoites (SM), and the protein levels in SM were significantly higher than in UO, SO, and SZ. The results of the indirect immunofluorescence localization showed that the protein was distributed mainly at the anterior region of SZ and on the surface and in the cytoplasm of SM. The fluorescence intensity increased further with its development in vitro. An anti-rEtANK polyclonal antibody inhibited the invasive ability of E. tenella in DF-1 cells. These results showed that EtANK may be related to host cell invasion, required for the parasite's growth in the host, and may be involved in the development of E. tenella resistance to some drugs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序