Analysis of phagocytosis by mIgM plus lymphocytes depending on monoclonal antibodies against IgM of largemouth bass (Micropterus salmoides)

文献类型： 外文期刊

第一作者： Wu, Jing

作者： Wu, Jing;Li, Yugu;Ma, Yanping;Hao, Le;Liu, Zhenxing;Ma, Yanping;Hao, Le;Liu, Zhenxing;Ma, Yanping;Hao, Le;Liu, Zhenxing;Nie, Yifan

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关键词： Largemouth bass; MAbs; IgM (+) lymphocytes; Phagocytosis; Fc gamma R

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 123 卷

页码：

收录情况： SCI

摘要： The phagocytic actives of B cells in fish have been proven in recent years. In this study, five positive hybridomas secreting monoclonal antibodies (MAbs) against largemouth bass IgM were produced. Indirect immunofluores-cence assay (IFA) demonstrated that five MAbs could specifically recognize membrane-bound IgM (mIgM) molecule of largemouth bass. Indirect ELISA and Western blotting analysis showed that all the five MAbs had no cross-reactions with the other two teleost IgMs. Flow cytometry analysis (FCM) revealed that the percentages of largemouth bass mIgM+ lymphocytes in head kidney, peripheral blood and spleen were 51.66 +/- 0.608%, 16.5 +/- 1.235% and 42.92 +/- 1.091%, respectively. In addition, the phagocytosis rates of mIgM + lymphocytes ingesting Nocardia seriolae from head kidney, peripheral blood and spleen were calculated to be 5.413 +/- 0.274%, 16.6 +/- 0.289% and 26.3 +/- 0.296%, respectively. The qPCR results of sorted cells indicated that most inflammatory cytokines (IFN gamma, IL-1 beta, IL-2, IL-12 beta, IL-34, IL-10), chemokine (CXCL12), chemokines receptors (CXCR2, CXCR4) and genes (Fc gamma RIa, NCF1, CFL, ARP2/3, CD45, Syk, MARCKS) related to Fc gamma R-mediated phagocytic signaling pathway in phagocytic mIgM+ lymphocytes were up-regulated significantly (P < 0.05). Taken together, the results suggested that the MAb (MM06H) produced in this paper could be used as a tool to study mIgM+ lym-phocytes of largemouth bass, and Fc gamma R may participate in the phagocytosis of mIgM+ lymphocytes, which is helpful to further study the role of mIgM+ lymphocytes in innate immunity.

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