Establishment of a Steatosis Model in LMH Cells, Chicken Embryo Hepatocytes, and Liver Tissues Based on a Mixture of Sodium Oleate and Palmitic Acid

文献类型： 外文期刊

第一作者： Zhuang, Wuchao

作者： Zhuang, Wuchao;Chen, Ziwei;Shu, Xin;Zhang, Jilong;Zhu, Runbang;Shen, Manman;Chen, Jianfei;Zheng, Xiaotong;Chen, Ziwei;Shu, Xin;Shen, Manman;Chen, Jianfei;Zheng, Xiaotong

作者机构：

关键词： fatty acid; RNA-seq; LMH; chicken embryo liver; lipid metabolism

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.0 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 15 期

页码：

收录情况： SCI

摘要： Simple Summary Establishing a cellular steatosis model is crucial for studying liver lipid deposition in poultry. This study used leghorn male hepatoma (LMH) cells to investigate the effects of oleic acid (OA), sodium oleate (SO), palmitic acid (PA), sodium palmitate (SP), and their pairwise combinations on steatosis development. Subsequently, RNA-seq was employed to comprehensively analyze alterations in gene expression within cells under various steatosis-inducing conditions. In primary chicken embryonic liver cells and liver tissue, the optimal conditions for inducing steatosis identified in this study can also induce steatosis effectively. Overall, this study found that a combination of SO and PA efficiently induces steatosis in various chicken liver cell types and chicken embryonic liver tissues.Abstract Research on hepatic steatosis in animal husbandry has been a prominent area of study. Developing an appropriate in vitro cellular steatosis model is crucial for comprehensively investigating the mechanisms involved in liver lipid deposition in poultry and for identifying potential interventions to address abnormalities in lipid metabolism. The research on the methods of in vitro liver steatosis in chickens, particularly the effects of different fat mixtures, is still lacking. In this study, LMH cells were utilized to investigate the effects of OA, SO, PA, SP, and their pairwise combinations on steatosis development, with the aim of identifying the optimal conditions for inducing steatosis. Analysis of triglyceride (TG) content in LMH cells revealed that OA and SP had limited efficacy in increasing TG content, while a combination of SO and PA in a 1:2 ratio exhibited the highest TG content. Moreover, Oil Red O staining results in LMH cells demonstrated that the combination treatment had a more pronounced induction effect compared to 0.375 mM SO. Additionally, RNA-seq analysis showed that 0.375 mM SO significantly influenced the expression of genes associated with fatty acid metabolism compared to the control group, whereas the combination of SO and PA led to an enrichment of key GO terms associated with programmed cell death. These findings suggest that varying conditions of cellular steatosis could lead to distinct disruptions in gene expression. The optimal conditions for inducing steatosis in LMH cells were also tested on chicken embryonic liver cells and embryos. TG detection and Oil Red O staining assays showed that the combination of SO and PA successfully induced steatosis. However, the gene expression pattern differed from that of LMH cells. This study lays the foundations for further investigations into avian hepatic steatosis.

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