A visual, rapid, and sensitive detection platform for Vibrio parahaemolyticus based on RPA-CRISPR/Cas12a and an immunochromatographic test strip

文献类型： 外文期刊

第一作者： Wang, Jinbin

作者： Wang, Jinbin;Liu, Hua;Zeng, Haijuan;Wang, Jinbin;Zeng, Haijuan;Xu, Danhong;Wu, Wenhui;Wang, Jinbin;Zhu, Lemei;Zeng, Haijuan;Zeng, Haijuan;Wu, Wenhui

作者机构：

关键词： CRISPR/Cas12a; immunochromatographic test strip; tlh; Vibrio parahaemolyticus

期刊名称：FOOD QUALITY AND SAFETY （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 2399-1399

年卷期： 2024 年 8 卷

页码：

收录情况： SCI

摘要： Objectives Vibrio parahaemolyticus is the primary species that causes vibriosis. In this study, a point-of-care detection method was developed for V. parahaemolyticus.Materials and Methods The detection platform targeted the thermolabile haemolysin (tlh) gene of V. parahaemolyticus based on recombinant polymerase amplification (RPA) and clustered regularly spaced short palindromic repeat (CRISPR/Cas) systems. The platform was combined with an immunochromatographic test strip (ICS) that enables low-cost, simple, visual detection of V. parahaemolyticus.Results The detection limit was 2.5x102 fg/mu L for plasmids and 1.4x102 CFU/mL for V. parahaemolyticus. In addition, V. parahaemolyticus in salmon sashimi could be detected at a concentration of 154 CFU/g without enrichment, and the entire detection time was around 30 min. After enrichment for 6 h, 2 CFU/g V. parahaemolyticus could be detected.Conclusions Consequently, the proposed RPA-CRISPR/Cas12a-ICS platform could detect V. parahaemolyticus in seafood intuitively, quickly, and sensitively, leading to high practical application value.

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