BmC/EBPZ gene is essential for the larval growth and development of silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Mei, Xinglin

作者： Mei, Xinglin;Huang, Tianchen;Liu, Weibin;Jiang, Li;Zhong, Shanshan;Shen, Dongxu;Qiao, Peitong;Zhao, Qiaoling;Chen, Anli;Shen, Dongxu;Zhao, Qiaoling

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关键词： Bombyx mori; BmC/EBPZ; GMS mutant; small larvae; positional cloning; CRISPR/Cas9

期刊名称：FRONTIERS IN PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.7 ）

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年卷期： 2024 年 15 卷

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收录情况： SCI

摘要： The genetic male sterile line (GMS) of the silkworm Bombyx mori is a recessive mutant that is naturally mutated from the wild-type 898WB strain. One of the major characteristics of the GMS mutant is its small larvae. Through positional cloning, candidate genes for the GMS mutant were located in a region approximately 800.5 kb long on the 24th linkage group of the silkworm. One of the genes was Bombyx mori CCAAT/enhancer-binding protein zeta (BmC/EBPZ), which is a member of the basic region-leucine zipper transcription factor family. Compared with the wild-type 898WB strain, the GMS mutant features a 9 bp insertion in the 3 ' end of open reading frame sequence of BmC/EBPZ gene. Moreover, the high expression level of the BmC/EBPZ gene in the testis suggests that the gene is involved in the regulation of reproduction-related genes. Using the CRISPR/Cas9-mediated knockout system, we found that the BmC/EBPZ knockout strains had the same phenotypes as the GMS mutant, that is, the larvae were small. However, the larvae of BmC/EBPZ knockout strains died during the development of the third instar. Therefore, the BmC/EBPZ gene was identified as the major gene responsible for GMS mutation.

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