Determination of Paralytic Shellfish Toxins in Bivalve Mollusks by Amino-Modified Multiwalled Carbon Nanotube (MWCNT) Solid-Phase Extraction (SPE) and High-Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)

文献类型： 外文期刊

第一作者： Wang, Xu-Feng

作者： Wang, Xu-Feng;Wang, Qiang;Zhao, Dong-Hao;Wang, Xu-Feng;Wang, Qiang;Zhao, Dong-Hao;Yang, Jin-Lan;Zhao, Dong-Hao

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关键词： Amino-modified multiwalled carbon nanotube (MWCNT-NH2); hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS; MS); paralytic shellfish toxins (PSTs); solid-phase extraction (SPE)

期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）

ISSN： 0003-2719

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收录情况： SCI

摘要： An amino-modified multiwalled carbon nanotube (MWCNT) solid-phase extraction-based hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) method was developed for simultaneous determination of 13 paralytic shellfish toxins (PSTs) in bivalve mollusks. Shellfish samples were extracted with 1% acetic acid followed by cleanup using optimized MWCNT-NH2 based solid-phase extraction. Absorbed PSTs were eluted with 2 mL 1% acetic acid-acetonitrile (70/30, v/v). The separation was performed on a Waters BEH Amide column within 7.5 min utilizing 0.1% formic acid-acetonitrile and 2 mM ammonium acetate containing 0.1% formic acid as the mobile phase. The analytes were quantified by matrix-matched external standard curves with correlation coefficients(R) exceeding 0.997 acquired in multiple reaction-monitoring mode. The limits of detection and quantitation in shellfish samples were from 2.76 to 9.8 mu g/kg and 9.11 to 39.3 mu g/kg, respectively. Recoveries at three spiked levels ranged from 71.4% to 97.8% with relative standard deviations less than 12.6%. The developed method was satisfactorily applied to the analysis of shellfish.

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