Functional Characterization of a Novel SMR-Type Efflux Pump RanQ, Mediating Quaternary Ammonium Compound Resistance in Riemerella anatipestifer

文献类型： 外文期刊

第一作者： Quan, Heng

作者： Quan, Heng;Liu, Donghui;Wang, Wenhui;Quan, Heng;Gong, Xiaowei;Chen, Qiwei;Zheng, Fuying;Yu, Yongfeng;Liu, Donghui;Chu, Yuefeng

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关键词： Riemerella anatipestifer; SMR efflux pump; quaternary ammonium compound; GE296_RS02355 gene; RanQ protein

期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）

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年卷期： 2023 年 11 卷 4 期

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收录情况： SCI

摘要： Riemerella anatipestifer (R. anatipestifer) is a multidrug-resistant bacterium and an important pathogen responsible for major economic losses in the duck industry. Our previous study revealed that the efflux pump is an important resistance mechanism of R. anatipestifer. Bioinformatics analysis indicated that the GE296_RS02355 gene (denoted here as RanQ), a putative small multidrug resistance (SMR)-type efflux pump, is highly conserved in R. anatipestifer strains and important for the multidrug resistance. In the present study, we characterized the GE296_RS02355 gene in R. anatipestifer strain LZ-01. First, the deletion strain RA-LZ01 Delta GE296_RS02355 and complemented strain RA-LZ01c Delta GE296_RS02355 were constructed. When compared with that of the wild-type (WT) strain RA-LZ01, the mutant strain Delta RanQ showed no significant influence on bacterial growth, virulence, invasion and adhesion, morphology biofilm formation ability, and glucose metabolism. In addition, the Delta RanQ mutant strain did not alter the drug resistance phenotype of the WT strain RA-LZ01 and displayed enhanced sensitivity toward structurally related quaternary ammonium compounds, such as benzalkonium chloride and methyl viologen, which show high efflux specificity and selectivity. This study may help elucidate the unprecedented biological functions of the SMR-type efflux pump in R. anatipestifer. Thus, if this determinant is horizontally transferred, it could cause the spread of quaternary ammonium compound resistance among bacterial species.

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