Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency

文献类型： 外文期刊

第一作者： Ban, Qian

作者： Ban, Qian;Duan, Quanchao;Zhang, Meng;Li, Xiaofeng;Shi, Zhenni;Zhang, Yan;Hou, Jinbin;Ye, Shoudong;Zhang, Baowei;Ban, Qian;Kang, Hanyue;Xu, Xiuzhen;Xu, Xiaobin;Hui, Wenqiao;Liu, Wenfei;Liu, Wenfei

作者机构：

期刊名称：ISCIENCE （ 影响因子：5.8； 五年影响因子：5.8 ）

ISSN：

年卷期： 2023 年 26 卷 4 期

页码：

收录情况： SCI

摘要： Here, we report the scalable fabrication of 2i-functionalized micro-pyramid-array (mu PyA/+2i) inserts for use in commercial multi-well plates, as the alternative culti-vation platform for maintaining long-term self-renewal and pluripotency of mul-tiple mESCs and mouse induced pluripotent stem cells. Relevant evidence including cell morphology characterization increased alkaline phosphatase activ-ity, high expression of mESC self-renewal markers, decreased levels of differen-tiation-associated markers, and high proportion of self-renewal marker cells are provided. Further studies demonstrated that mu PyA/+2i could cause a higher cell density in mESC colony, and induce gene expression changes. Subsequent studies showed that mu PyA/+2i can influence the cytoskeleton and promote cell adhesion through Cldn-7 upregulation. In summary, these mu PyA/+2i inserts offer flexible and gelatin-free micro-envriomnets to maintain long-term self-renewal and pluripotency of mESCs. Enabled by the microstructured inserst, the facile stem cell manipulation and transfer among culture dishes will broaden stem cells both in routine and translational applications.

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