CsAP2-09 confers resistance against citrus bacterial canker by regulating CsGH3.1L-mediated phytohormone biosynthesis

文献类型： 外文期刊

第一作者： Fu, Jia

作者： Fu, Jia;Yu, Qiyuan;Zhang, Chenxi;Xian, Baohang;Fan, Jie;Huang, Xin;Yang, Wen;Zou, Xiuping;Chen, Shanchun;He, Yongrui;Li, Qiang;Zou, Xiuping;Chen, Shanchun;He, Yongrui;Li, Qiang;Zou, Xiuping;Chen, Shanchun;He, Yongrui;Li, Qiang;Su, Liyan

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关键词： Apetala 2; ethylene response factor (AP2; ERF); Citrus bacterial canker (CBC); Gretchen hagen 3 (GH3); CsGH3; 1L

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2023 年 229 卷

页码：

收录情况： SCI

摘要： Citrus bacterial canker (CBC) is a serious bacterial disease affecting citrus plantations and the citrus industry all over the world. We have previously shown that an apetala 2/ethylene response factor in Citrus sinensis, CsAP2-09, positively regulated resistance to CBC, although the regulatory mechanisms remained undetermined. Here, we demonstrated that CsAP2-09 positively and sustainably controlled resistance to CBC in three-year transgenic plants. CsAP2-09 was found to be a transcriptional activator, and qRT-PCR and dual luciferase assays showed that it controlled the expression CsGH3.1L. CsAP2-09 bound directly to the promotor of CsGH3.1L, shown by yeast one-hybrid assay, with the binding site confirmed by electrophoretic mobility shift assay. Biochemical assays showed that CsAP2-09 negatively regulated the biosynthesis of indole acetic acid (IAA) and positively regulated that of salicylic acid (SA) and ethylene, verified with transient overexpression of CsGH3.1L. The combination of these results with those of previous reports indicated that SA, ethylene, and IAA can directly regulate CBC resistance. Overall, we revealed a pathway whereby CsAP2-09 conferred CBC resistance by direct binding to the CsGH3.1L promoter, activating its expression and modulating IAA, SA, and ethylene biosynthesis. Our study indicates the potential value of manipulating CsAP2-09 and CsGH3.1L in the breeding of CBC-resistant citrus.

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