Generation and evaluation of Salmonella entericaserovar Choleraesuis mutant strains as a potential live-attenuated vaccine

文献类型： 外文期刊

第一作者： Huang, Xin

作者： Huang, Xin;Yang, Shanshan;Zhao, Jing;Yang, Jing;Jiang, Huazheng;Li, Shuxian;Wang, Caiying;Liu, Guangliang;Yang, Shanshan;Yang, Jing;Li, Shuxian;Liu, Guangliang;Wang, Caiying

作者机构：

关键词： Salmonella enterica serovar Choleraesuis; CRISPR-Cas9; rpoS; slyA; Mucosal immunity

期刊名称：VACCINE （ 影响因子：3.5； 五年影响因子：3.5 ）

ISSN： 0264-410X

年卷期： 2024 年 42 卷 24 期

页码：

收录情况： SCI

摘要： Background: Salmonella entericaserovar Choleraesuis (S. C) is a swine enteric pathogen causing paratyphoid fever, enterocolitis, and septicemia in piglets. S. C is mainly transmitted through the fecal-oral route. Vaccination is an effective strategy for preventing and controlling Salmonella infection. Results: Herein, we used CRISPR-Cas9 technology to knockout the virulence regulatory genes, rpoS, and slyA of S. C and constructed the drpoS, dslyA, and drpoS dslyA strains. The phenotypic characteristics of the mutant strains remained unchanged compared with the parental wild-type strain. In vivo study, unlike the wild-type strain, the dslyA and drpoS dslyA strains alleviated splenomegaly, colon atrophy, and lower bacterial loads in the spleen, liver, ileum, and colon. These mutant strains survived in Peyer's patches (PPs) and mesenteric lymph nodes (MLN) for up to 15 days post-infection. Furthermore, the immunization of the drpoS dslyA strain induced robust humoral and cellular immune responses. Conclusions: Consequently, vaccination with drpoS dslyA conferred a high percentage of protection against lethal invasive Salmonella, specifically S. C, in mice. This study provided novel insights into the development of live-attenuated vaccines against the infection of S. C.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序