An intelligent sensing platform for sugarcane pokkah boeng disease based on CHA-driven RCA cascade DNAzyme amplification technology and clustered DNA nanoflowers

文献类型： 外文期刊

第一作者： Wu, Qingnian

作者： Wu, Qingnian;Song, Yujie;Jin, Chenchen;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu;Wang, Zeping;Liao, Jie;Yan, Feiyan

作者机构：

关键词： Sugarcane pokkah boeng disease; Cascade amplification technology; Bundle DNA nanoflowers; Smartphone assisted detection

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.7； 五年影响因子：7.4 ）

ISSN：

年卷期： 2025 年 428 卷

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收录情况： SCI

摘要： Sugarcane pokkah boeng disease poses a significant threat to global sugarcane production, underscoring the urgent need for a sensitive and user-friendly detection method. This study introduces a biosensing platform that harnesses CHA-driven rolling circle amplification (RCA), DNAzyme amplification technology, and clustered DNA nanoflowers for the precise detection of the disease. The smartphone-assisted platform is designed for simplicity and intelligent operation. The nanoflower-like DNA structure efficiently increases the bioelectrode surface area, which enhances the detection sensitivity. By employing CHA-driven RCA and DNAzyme cascade amplification, the platform generates a substantial amount of output DNA that binds to the self-stacking nanoflowering DNA on the biological cathode. The addition of a capacitor in series with the detection platform further enhances the sensitivity of biosensor by 6.7 times. The platform exhibits a strong linear response to target concentrations ranging from 0.0001 pM to 10,000 pM, with a detection limit of 6.5 x 10- 2 fM (S/N = 3). When applied to real samples, the results of platform are closely matched to those of fluorescence quantitative PCR, demonstrating its practical application and potential for further development.

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