Metabolomics reveals the mechanism of tetracycline biodegradation by a Sphingobacterium mizutaii S121

文献类型： 外文期刊

第一作者： Tan, Hao

作者： Tan, Hao;Kong, Delong;Li, Qingqing;Zhou, Yiqing;Jiang, Xu;Ruan, Zhiyong;Tan, Hao;Wang, Zhiye;Parales, Rebecca E.;Ruan, Zhiyong;Ruan, Zhiyong

作者机构：

关键词： Tetracycline; Sphingobacterium mizutaii S121; Response surface methodology (RSM); Biodegradation products; Nontargeted metabolomics

期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：9.988； 五年影响因子：10.366 ）

ISSN： 0269-7491

年卷期： 2022 年 305 卷

页码：

收录情况： SCI

摘要： Contamination by tetracycline residues has adverse influences on the environment and is considered a pressing issue. Biodegradation is regarded as a promising way to treat tetracycline residues in the environment. Here, strain Sphingobacterium mizutaii S121, which could degrade 20 mg/L tetracycline completely within 5 days, was isolated from contaminated soil. The characteristics of tetracycline degradation by strain S121 were investigated under various culture conditions. Response surface methodology was used to predict the maximum tetracycline degradation ratio, which can be obtained under the following conditions: 31.36 C, pH of 7.15, and inoculum volume of 5.5% (v/v). Furthermore, extracellular tetracycline biodegradation products and intracellular metabolic pathways of S121 were detected by ultraperformance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and UHPLC-quadrupole electrospray (QE)-MS, respectively. The results identified eight possible degradation products, and three putative degradation pathways were proposed. In addition, exposure to tetracycline produced significant influences on metabolic pathways such as pyrimidine, purine, taurine and hypotaurine metabolism and lysine degradation. Consequently, the intracellular metabolic pathway response of S121 in the presence of tetracycline was proposed. These findings are presented for the first time, which will facilitate a comprehensive understanding of the mechanism of tetracycline degradation. Moreover, strain S121 can be a promising bacterium for tetracycline bioremediation.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序