Cloning and characterization of the lysis protein LysSGF3 from Shigella Microviridae phage SGF3 for control of pathogenic bacteria and biofilms

文献类型： 外文期刊

第一作者： Chang, Zhankun

作者： Chang, Zhankun;Liu, Bingxin;Guo, Qiucui;Li, Zong;Liu, Ruyin;Liu, Xinchun;Lu, Han

作者机构：

关键词： Shigella flexneri; Microviridae; Lysis protein; Protein expression; Bactericidal activity

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 316 卷

页码：

收录情况： SCI

摘要： Endolysins (lysins) are novel bactericidal agents derived from phages. In contrast, single-stranded DNA (ssDNA) phages can lyse host bacteria via an isolated lysis protein, with improved efficiency and speed compared to the binary lysis system "lysin-holin" employed by double-stranded DNA (dsDNA) phages. In this study, three uncharacterized proteins from Shigella Microviridae phage SGF3 were cloned and expressed. From them, a novel lysis protein, LysSGF3, was identified, and factors influencing its bactericidal activity and properties were investigated. These features were applied to biofilm control. LysSGF3 at a concentration of 100 mu g/mL exhibited robust lysis activity of 76.13 % against S. flexneri 1.10599 at 37 degrees C and pH = 7. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of LysSGF3 on S. flexneri 1.10599 were 50 mu g/ mL and 300 mu g/mL, respectively. Moreover, LysSGF3 exhibited a wider lysis spectrum than phage SGF3, with robust effects against 11 Gram-negative strains of Shigella, Escherichia coli, and Edwardsiella and three Grampositive strains including Staphylococcus aureus and Bacillus. The biofilm formed by strains of Shigella, E. coli, and S. aureus experienced a removal rate of at least 50 %. At the same time, the combined biofilm of S. flexneri 1.10599 and other strains also experienced adequate removal. In summary, LysSGF3 is a promising new bactericidal agent that will provide alternative solutions to overcome antibiotic resistance.

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