Identification and functional characterization of a cystathionine beta-lyase (CBL) enzyme for H2S production in Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Wang, Zhiqing

作者： Wang, Zhiqing;He, Feng;Zhang, Liping;Liu, Zhiqiang;Liu, Danmei;Jin, Zhuping;Pei, Yanxi;He, Feng;Mu, Yao;Yang, Jinbao;Jin, Zhuping

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关键词： Cystathionine ?-lyase; Hydrogen sulfide; Abiotic stress; Arabidopsis thaliana

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.437； 五年影响因子：5.731 ）

ISSN： 0981-9428

年卷期： 2022 年 182 卷

页码：

收录情况： SCI

摘要： Sulfide or sulfur metabolism plays an important role in the growth and development of plants. Cystathionine beta-lyase (CBL) is an important enzyme in methionine synthesis, but a comprehensive understanding of CBL functions is limited. As the third gasotransmitter, hydrogen sulfide (H2S) plays important physiological roles in plants. In this study, we found that the endogenous H2S content in Arabidopsis thaliana cbl mutants was lower than that in the wild type. Under PEG-based osmotic stress conditions, the H2S contents of CBL-overexpression (OE-CBL) plants increased significantly compared with the wild type. Additionally, the OE-CBL plants increased their tolerance to osmotic stress by increasing the transcription levels of drought-related genes and their relative water-loss rates. Compared with cbl and wild type, OE-CBL plants resisted drought stress by significantly closing their stomata, resulting in improved survival rates. Root tip-bending experiments showed that CBL over expression relieved osmotic, heavy metal and cold stresses in Arabidopsis. The recombinant CBL activity in vitro revealed that CBL produced H2S using L-cysteine as a substrate. Thus, CBL had a very strong cysteine desulfhydrase activity that could produce endogenous H2S using L-cysteine as a substrate, and it played an important role in plant abiotic stress resistance.

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