Sheep-derived butyrate-producing Clostridium beijerinckii R8 alleviates diarrhea by shaping the gut microbiota of goat kids

文献类型： 外文期刊

第一作者： Fan, Dingkun

作者： Fan, Dingkun;Fu, Yuze;Zhang, Jixian;Bi, Yanliang;Ma, Tao;Diao, Qiyu;Zhang, Naifeng

作者机构：

关键词： Clostridium beijerinckii; Diarrhea; Intestinal microbiota; Intestinal immune; Intestinal barrier

期刊名称：ANIMAL NUTRITION （ 影响因子：7.5； 五年影响因子：7.3 ）

ISSN： 2405-6383

年卷期： 2024 年 19 卷

页码：

收录情况： SCI

摘要： Newborn goat kids exposed to environmental stress are susceptible to diarrhea due to immature intestinal functions and undeveloped gut microbiota. Butyrate-producing bacteria as next generation probiotics benefit the maintenance of intestinal health, but the mode of regulation is still unclear. Herein, a novel butyrate-producing strain was isolated from sheep rumen and identified as Clostridium beijerinckii (C. beijerinckii) R8, thereafter goat kids were treated with C. beijerinckii R8 to elucidate its regulatory mechanisms on diarrhea. Thirty-six goat kids were assigned to four groups: control (CON), low dose (LCB; supplementation with 5 x 10(7) CFU/mL of C. beijerinckii R8, each with 10 mL/d), middle dose (MCB; supplementation with 5 x 10(8) CFU/mL of C. beijerinckii R8, each with 10 mL/d), high dose (HCB; supplementation with 5 x 10(9) CFU/mL of C. beijerinckii R8, each with 10 mL/d). The experiment lasted for 15 d, and 6 goat kids were randomly selected from each group for slaughter on the last day of the trial. The results showed that this isolate reduced the diarrhea rate (P < 0.001) and fecal scores (P < 0.001). In the gut, its supplementation inhibited inflammation, increased antioxidant capacity, and regulated intestinal flora. Meanwhile, C. beijerinckii R8 strengthened the intestinal barrier and altered the jejunum morphology. This evidence suggests that C. beijerinckii R8 may alleviate diarrhea in goat kids by regulating microbiota, which is directly related to intestinal barrier and immune capacity.

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