Screening and identification of the dominant antigens of the African swine fever virus

文献类型： 外文期刊

第一作者： Xu, Zhaoyang

作者： Xu, Zhaoyang;Hu, Yifan;Li, Junbo;Wang, Ancheng;Meng, Xin;Chen, Lingchao;Wei, Jianchao;Tong, Wu;Kong, Ning;Yu, Lingxue;Yu, Hai;Shan, Tongling;Tong, Guangzhi;Zheng, Hao;Xu, Zhaoyang;Wang, Guihua;Li, Junbo;Meng, Xin;Zheng, Hao

作者机构：

关键词： African swine fever virus; dominant antigen; p30 protein; p54 protein; p22 protein

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.2； 五年影响因子：3.5 ）

ISSN：

年卷期： 2023 年 10 卷

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收录情况： SCI

摘要： African swine fever is a highly lethal contagious disease of pigs for which there is no vaccine. Its causative agent African swine fever virus (ASFV) is a highly complex enveloped DNA virus encoding more than 150 open reading frames. The antigenicity of ASFV is still unclear at present. In this study, 35 proteins of ASFV were expressed by Escherichia coli, and ELISA was developed for the detection of antibodies against these proteins. p30, p54, and p22 were presented as the major antigens of ASFV, positively reacting with all five clinical ASFV-positive pig sera, and 10 pig sera experimentally infected by ASFV. Five proteins (pB475L, pC129R, pE199L, pE184L, and pK145R) reacted well with ASFV-positive sera. The p30 induced a rapid and strong antibody immune response during ASFV infection. These results will promote the development of subunit vaccines and serum diagnostic methods against ASFV.

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