Babeisa duncani infection alters gut microbiota profile in hamsters

文献类型： 外文期刊

第一作者： Zhang, Shangdi

作者： Zhang, Shangdi;Wang, Yanbo;Nian, Yueli;You, Chongge;Zhang, Dekui;Wang, Jinming;Li, Xiaoyun;Guan, Guiquan

作者机构：

关键词： Babesia duncani; gut microbiota; human babesiosis; biomarker; 16S rRNA gene

期刊名称：PARASITES HOSTS AND DISEASES

ISSN： 2982-5164

年卷期： 2023 年 61 卷 1 期

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收录情况： SCI

摘要： The genus Babesia includes parasites that can induce human and animal babesiosis, which are common in tropical and subtropical regions of the world. The gut microbiota has not been examined in hamsters infected by Babesia duncani. Red blood cells infect-ed with B. duncani were injected into hamsters through intraperitoneal route. To evaluate the changes in gut microbiota, DNAs were extracted from small intestinal contents, ac-quired from hamsters during disease development. Then, the V4 region of the 16S rRNA gene of bacteria was sequenced using the Illumina sequencing platform. Gut microbiota alternation and composition were assessed according to the sequencing data, which were clustered with >97.0% sequence similarity to create amplicon sequence variants (ASVs). Bacteroidetes and Firmicutes were made up of the major components of the gut microbiota in all samples. The abundance of Bacteroidetes elevated after B. duncani in-fection than the B. duncani-free group, while Firmicutes and Desulfobacterota declined. Alpha diversity analysis demonstrated that the shown ASVs were substantially decreased in the highest parasitemia group than B. duncani-free and lower parasitemia groups. Po-tential biomarkers were discovered by Linear discriminant analysis Effect Size (LEfSe) analysis, which demonstrated that several bacterial families (including Muribaculaceae, Desulfovibrionaceae, Oscillospiraceae, Helicobacteraceae, Clostridia UGG014, Desulfo-vibrionaceae, and Lachnospiraceae) were potential biomarkers in B. duncani-infected hamsters. This research demonstrated that B. duncani infectious can modify the gut mi-crobiota of hamsters.

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