Development of an efficient plant dual cytosine and adenine editor

文献类型： 外文期刊

第一作者： Xu, Rongfang

作者： Xu, Rongfang;Kong, Fanna;Qin, Ruiying;Li, Juan;Liu, Xiaoshuang;Wei, Pengcheng;Liu, Xiaoshuang;Wei, Pengcheng

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关键词： base editing; CRISPR; dual-base editor; genome editing; rice

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2021 年 63 卷 9 期

页码：

收录情况： SCI

摘要： An enhanced CDA-like (eCDAL) was established from Japanese lamprey CDA1-like 4 to achieve a high editing frequency in a broad region as a C-terminal cytosine base editors (CT-CBE). Then, a novel plant dual-base editor version 1(pDuBE1) was developed by integrating TadA-8e into eCDAL. The editing efficiency of pDuBE1 could reach to 87.6%, with frequencies of concurrent A-to-G and C-to-T conversions as high as 49.7% in stably transformed plant cells. Our results showed that pDuBE1 could mediate robust dual editing in plant genome, providing a powerful manipulation tool for precise crop breeding and screening platforms for in planta direct evolution.

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