Impairment of testes development in Yangzhou ganders by augmentation of leptin receptor signaling

文献类型： 外文期刊

第一作者： Lei, M. M.

作者： Lei, M. M.;Dai, Z. C.;Zhu, H. X.;Chen, R.;Chen, Z.;Shao, C. R.;Shi, Z. D.;Lei, M. M.;Dai, Z. C.;Zhu, H. X.;Chen, R.;Chen, Z.;Shao, C. R.;Shi, Z. D.

作者机构：

关键词： Ganders; Leptin receptor; Testicular development; Testosterone; Molecular mechanisms

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN： 0093-691X

年卷期： 2021 年 171 卷

页码：

收录情况： SCI

摘要： The aim of this study was to determine the cellular and molecular mechanisms of leptin (LEP) and the leptin receptor (LEPR) in testicular development of prepubertal ganders. In an in vivo animal experiment, active immunization against LEPR severely depressed prepubertal testicular development by significantly reducing testicular weights at 200 and 227 days of age. The number of elongated spermatids in the seminiferous tubules was also significantly decreased by immunization with LEPR at ages of 200 and 227 days. Inhibition of testicular development by LEPR immunization was associated with decreases in LHR, StAR, 3 beta-HSD, CYP11A1, CYP17A1, and PRLR mRNA expression levels in testicular tissue, which resulted in a significant decrease in testosterone synthesis. In the in vitro experiments, the addition of LEP combined with anti-LEPR antibodies strengthened LEPR signal transduction, and inhibited significantly testosterone production in cultured Leydig cells isolated from prepubertal gander testes. The mRNA expression of LHR, StAR, 3 beta-HSD, CYP11A1, CYP17A1 also decreased significantly after treatment with LEP combined with anti-LEPR antibodies in cultured Leydig cells. These results suggest that anti-LEPR antibodies strengthen LEPR signaling transduction in the presence of LEP, and immunization against LEPR inhibited testes development and testosterone secretion in prepubertal ganders. (C) 2021 Elsevier Inc. All rights reserved.

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