Trans-cinnamaldehyde inhibits Escherichia coli growth by regulating lipopolysaccharide accumulation

文献类型： 外文期刊

第一作者： Xing, Huanling

作者： Xing, Huanling;Liu, Xiaomin;Lin, Jianhao;Huang, Junyi;Li, Xinghai;Li, Yanqun;Guo, Shining;Wu, Hong;Wu, Hong;Sun, Mingfei;Zhou, Fang

作者机构：

关键词： Trans-cinnamaldehyde; Resistant Escherichia coli; Susceptible Escherichia coli; Cell wall; LPS

期刊名称：FOOD BIOSCIENCE （ 影响因子：4.8； 五年影响因子：5.1 ）

ISSN： 2212-4292

年卷期： 2024 年 61 卷

页码：

收录情况： SCI

摘要： Colibacillosis is among the most commonly occurring and economically devastating bacterial diseases of poultry worldwide owing to the misuse and overuse of antibiotics. Trans-cinnamaldehyde exhibits effective inhibition on multidrug-resistant Escherichia coli by disrupting the integrity of its cell wall. However, the mechanism underlying this inhibition is unclear. We found that trans-cinnamaldehyde inhibited E. coli efficiently with a minimum inhibitory concentration (MIC) of 140 mu g/mL. Trans-cinnamaldehyde disrupted the integrity of the E. coli cell wall due to an imbalance between lipopolysaccharide (LPS) and phospholipids. The structural change in the cell wall disrupted its stability, further causing the leakage of alkaline phosphatase (AKP) and beta-galactosidase, proteins, and DNA/RNA, and altering biofilms in the E. coli CVCC 1567 and the E. coli (047). This finally resulted in the death of E. coli. Inhibition by trans-cinnamaldehyde was more significant on the wall of E. coli CVCC 1567 because of a more severe loss of high molecular weight proteins and an easier dissolution of its cells. Transcinnamaldehyde affected gene expression of enzymes regulating LPS synthesis (lpxC, lpxA, lpxK, lpxM, yejM, ftsH, lapB), ultimately altering (LPS) content. Indeed, this effect is particularly pronounced in the genes expression of E. coli (047). In conclusion, trans-cinnamaldehyde is a potential novel drug candidate for preventing and treating infectious diseases caused by resistant E. coli clinical strains.

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