Development of sensitive and stable electrochemical impedimetric biosensor based on T1R1 receptor and its application to detection of umami substances

文献类型： 外文期刊

第一作者： Wang, Yijian

作者： Wang, Yijian;Kong, Liqin;Shu, Guoqiang;Sun, Guanqquan;Feng, Yaoze;Zhu, Ming;Wang, Yijian;Kong, Liqin;Shu, Guoqiang;Sun, Guanqquan;Feng, Yaoze;Zhu, Ming;Wang, Yijian;Feng, Yaoze;Feng, Yaoze;Kong, Liqin;Sun, Guanqquan;Feng, Yaoze;Zhu, Ming

作者机构：

关键词： Umami; Electrochemical biosensor; Electrochemical impedance spectroscopy

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 423 卷

页码：

收录情况： SCI

摘要： Umami substances can provide a palatable flavour for food. In this study, an electrochemical impedimetric biosensor was developed for detecting umami substances. This biosensor was fabricated by immobilising T1R1 onto AuNPs/reduced graphene oxide/chitosan which was in advance electro-deposited onto a glassy carbon electrode. The evaluation by the electrochemical impedance spectrum method showed that the T1R1 biosensor performed well with low detection limits and wide linear ranges. Under the optimised incubation time (60 s), the electrochemical response was linearly related to the concentrations of the detected targets monosodium gluta-mate and inosine-5 '-monophosphate within their respective linear range of 10-14 to 10-9 M and 10-16 to 10-13 M. The low detection limit of monosodium glutamate and inosine-5 '-monophosphate was 10-15 M and 10-16 M, respectively. Moreover, the T1R1 biosensor exhibited high specificity to umami substances even in the real food sample. The developed biosensor still retained 89.24% signal intensity after 6-day storage, exhibiting a desirable storability.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序