OSNRT1.1B-OSCNGC14/16-CA2+-OSNLP3 Pathway: Phosphorylation-Mediated Maintenance of Nitrogen Homeostasis

文献类型： 外文期刊

第一作者： Wang, Xiaohan

作者： Wang, Xiaohan;Liu, Yongqiang;Li, Weiwei;Wang, Yiqin;Chu, Chengcai;Liu, Yongqiang;Ma, Xiaojun;Wang, Wei;Jiang, Zhimin;Hu, Bin;Chu, Chengcai;Ma, Xiaojun;Wang, Wei;Jiang, Zhimin;Hu, Bin;Chu, Chengcai;Li, Weiwei;Ma, Xiaojun;Wang, Wei;Jiang, Zhimin;Hu, Bin;Chu, Chengcai;Li, Legong

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关键词： Ca2(+) signaling; nitrate Signaling; nitrogen homeostasis; phosphorylation; rice

期刊名称：ADVANCED SCIENCE （ 影响因子：14.1； 五年影响因子：15.6 ）

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年卷期： 2025 年

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收录情况： SCI

摘要： Nitrate, a crucial nutrient and signaling molecule, is extensively studied across plants. While the NRT1.1-NLP-centered pathway dominates nitrate signaling in Arabidopsis and rice, however, whether there is functional interaction or co-regulation between the primary nitrate response (PNR) and long-term nitrogen utilization remains unclear. Here, a novel nitrate signaling pathway is identified in rice that works alongside the established ubiquitination-mediated OsNRT1.1B-OsSPX4-OsNLP3 cascade. It is demonstrated that OsCNGC14, OsCNGC16, and OsNRT1.1B form a plasma membrane-localized complex in root tips, mediating nitrate-triggered Ca2(+) influx. The absence of either OsCNGC14 or OsCNGC16 abolished Ca2(+) signaling and suppressed PNR. The OsNRT1.1B-OsCNGC14/16 complex activates Ca2(+)-dependent phosphorylation of OsNLP3 at Ser193, which accelerates its nuclear translocation and transcriptional activation of nitrate-responsive genes. This phosphorylation enhances both short-term PNR and long-term nitrogenutilization. This findings reveal a dual regulatory network in rice: the Ca2(+)-OsNLP3 pathway rapidly amplifies nitrate signals, while the ubiquitination-mediated OsSPX4 degradation ensures sustained nitrogen homeostasis.

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