Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak

文献类型： 外文期刊

第一作者： La, Yongfu

作者： La, Yongfu;Li, Zhongbang;Ma, Xiaoming;Bao, Pengjia;Chu, Min;Guo, Xian;Liang, Chunnian;Yan, Ping;La, Yongfu;Li, Zhongbang;Ma, Xiaoming;Bao, Pengjia;Chu, Min;Guo, Xian;Liang, Chunnian;Yan, Ping;La, Yongfu;Li, Zhongbang;Ma, Xiaoming;Bao, Pengjia;Chu, Min;Guo, Xian;Liang, Chunnian;Yan, Ping

作者机构：

关键词： Yak; testis; lysozyme-like (LYZL) proteins; proliferating cell nuclear antigen

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：3.7； 五年影响因子：3.3 ）

ISSN： 1049-5398

年卷期： 2024 年 35 卷 1 期

页码：

收录情况： SCI

摘要： Lysozyme like 4 (LYZL4), lysozyme like 6 (LYZL6) and proliferating cell nuclear antigen (PCNA) are implicated in the regulation of testicular function, but there was no research reported available on the expression patterns of LYZL4, LYZL6 and PCNA genes at different developmental stages of yak testes. In this study, we used the qRT-PCR, western blotting and immunohistochemistry estimated the LYZL4, LYZL6 and PCNA gene expression and protein lo-calization at different developmental stages of yak testes. The qPCR results showed that the mRNA expression of LYZL4, LYZL6 and PCNA genes significantly increased with age in the testes of yaks. Western blot results showed that the protein abundance of LYZL4, LYZL6 and PCNA in yak testes was significantly higher after puberty than before puberty. Furthermore, the results of immunohistochemistry indicated that LYZL4, LYZL6 and PCNA may be involved in the regulation of spermatogonia proliferation and Leydig cell function in immature testis. In adult yak testes, LYZL4, LYZL6 and PCNA may involve in the development of round spermatids and primary spermatocytes during testicular development. Our results indicated that LYZL4, LYZL6 and PCNA may be involved in the development of Sertoli cells, Leydig cells and gonocytes in yak testes.

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