A xylanase with broad pH and temperature adaptability from Streptomyces megasporus DSM 41476, and its potential application in brewing industry

文献类型： 外文期刊

第一作者： Qiu, Zhenhua

作者： Qiu, Zhenhua;Shi, Pengjun;Luo, Huiying;Bai, Yingguo;Yuan, Tiezheng;Yang, Peilong;Yao, Bin;Qiu, Zhenhua;Liu, Suchun

作者机构：

关键词： Xylanase;Streptomyces megasporus;Yeast expression;Mashing

期刊名称：ENZYME AND MICROBIAL TECHNOLOGY （ 影响因子：3.493； 五年影响因子：3.699 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A xylanase gene, xynAM6, was isolated from the genomic DNA library of Streptomyces megasporus DSM 41476 using colony PCR screening method. The 1440-bp full-length gene encodes a 479-amino acid peptide consisting of a putative signal peptide of 36 residues, a family 10 glycoside hydrolase domain and a family 2 carbohydrate-binding module. The mature peptide of xynAM6 was successfully expressed in Pichia pastoris GS115. The optimal pH and temperature were pH 5.5 and 70 °C, respectively. The enzyme showed broad temperature adaptability (>60% of the maximum activity at 50-80 °C), had good thermostability at 60 °C and 70 °C, remained stable at pH 4.0-11.0, and was resistant to most proteases. The K_m and V_(max) values for oat spelt xylan were 1.68 mg ml~(-1) and 436.76 μmol min~(-1) mg~(-1), respectively, and 2.33 mgml~(-1) and 406.93 μmol min~(-1) mg~(-1) for birchwood xylan, respectively. The hydrolysis products of XYNAM6 were mainly xylose and xylobiose. Addition of XYNAM6 (80 U) to the brewery mash significantly reduced the filtration rate and viscosity by 36.33% and 35.51%, respectively. These favorable properties probably make XYNAM6 a good candidate for application in brewing industry.

分类号： Q5

  • 相关文献

[1]A new xylanase from Streptomyces megasporus DSM 41476 with high yield of xylobiose. Shi, Pengjun,Qiu, Zhenhua,Bai, Yingguo,Yuan, Tiezheng,Huang, Huoqing,Pan, Xia,Yang, Peilong,Yao, Bin,Zhang, Wei. 2012

[2]Comparison of the Delta(12) fatty acid desaturase gene between high-oleic and normal-oleic peanut genotypes. Yu, Shanlin,Pan, Lijuan,Zhang, Hongsheng,Pan, Lijuan,Yang, Qingli,Min, Ping,Ren, Zengkai. 2008

[3]Enhanced expression of the E-rns protein of classical swine fever virus in yeast and its application in an indirect enzyme-linked immunosorbent assay for antibody differentiation of infected from vaccinated animals. Luo, Yuzi,Li, Lin,Dong, Mei,Xu, Jingjing,Shao, Lina,Lei, Jianlin,Li, Na,He, Wen-Rui,Zhao, Bibo,Li, Su,Li, Yongfeng,Sun, Yuan,Qiu, Hua-Ji,Austermann-Busch, Sophia,Becher, Paul,Liu, Lihong,Luo, Yuzi,Li, Lin,Xu, Jingjing,Shao, Lina,Lei, Jianlin,He, Wen-Rui,Zhao, Bibo,Li, Su,Li, Yongfeng,Liu, Lihong,Sun, Yuan,Qiu, Hua-Ji.

[4]Isolation and characterization of an oilseed rape MAP kinase BnMPK3 involved in diverse environmental stresses. Yu, SW,Zhang, LD,Zuo, KJ,Tang, DQ,Tang, KX.

[5]Isolation and Functional Characterization of a Novel Gene Encoding a Dehydration Responsive Element Binding Transcription Factor from Populus euphratica. Wei, Li,Wang, Suomin,Ma, Jiangtao,Wu, Yanmin,Ma, Jiangtao.

[6]The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides. Chen, F.,Diao, Y.,Liu, X.,Fan, J.,Chen, F.,Cools, H. J.,Kelly, S. L.,Kelly, S. L.. 2014

[7]ZmDBF3, a Novel Transcription Factor from Maize (Zea mays L.), Is Involved in Multiple Abiotic Stress Tolerance. Zhou, Wei,Jia, Cheng-Guo,Wu, Xian,Yu, Gang,Zhang, Xiang-Hui,Liu, Jin-Liang,Pan, Hong-Yu,Zhou, Wei,Hu, Rui-Xue,Wu, Xian.

[8]Symbiotic Streptomyces sp. TN119 GH 11 xylanase: a new pH-stable, protease- and SDS-resistant xylanase. Zhou, Junpei,Shi, Pengjun,Zhang, Rui,Huang, Huoqing,Meng, Kun,Yang, Peilong,Yao, Bin. 2011

[9]Isolation and Identification of a Newly Isolated Alternaria sp ND-16 and Characterization of Xylanase. Li, Yin,Liu, Zhiqiang,Liu, Zhiqiang,Li, Geng,Cui, Fengjie,Ping, Lifeng,Qiu, Chongyan,Yan, Lijiao,Yan, Lijiao. 2009

[10]An acid and highly thermostable xylanase from Phialophora sp G5. Zhang, Fan,Shi, Pengjun,Bai, Yingguo,Luo, Huiying,Yuan, Tiezheng,Huang, Huoqing,Yang, Peilong,Yao, Bin,Zhang, Fan,Miao, Lihong. 2011

[11]Cloning, Expression, and Characterization of a New Streptomyces sp S27 Xylanase for Which Xylobiose is the Main Hydrolysis Product. Li, Ning,Shi, Pengjun,Yang, Peilong,Wang, Yaru,Luo, Huiying,Bai, Yingguo,Zhou, Zhigang,Yao, Bin. 2009

[12]Two Family 11 Xylanases from Achaetomium sp Xz-8 with High Catalytic Efficiency and Application Potentials in the Brewing Industry. Zhao, Liang,Meng, Kun,Bai, Yingguo,Shi, Pengjun,Huang, Huoqing,Luo, Huiying,Wang, Yaru,Yang, Peilong,Yao, Bin,Zhao, Liang,Song, Wei. 2013

[13]Cloning of a New Xylanase Gene from Streptomyces sp TN119 Using a Modified Thermal Asymmetric Interlaced-PCR Specific for GC-Rich Genes and Biochemical Characterization. Zhou, Junpei,Huang, Huoqing,Meng, Kun,Shi, Pengjun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Bai, Yingguo,Yao, Bin. 2010

[14]Gene Cloning, Overexpression, and Characterization of a Xylanase from Penicillium sp CGMCC 1669. Liu, Wanli,Shi, Pengjun,Yang, Peilong,Wang, Guozeng,Wang, Yaru,Luo, Huiying,Yao, Bin,Liu, Wanli,Chen, Qiang. 2010

[15]Purification and characterization of a xylanase from Bacillus subtilis isolated from the degumming line. Guo, Gang,Liu, Zhengchu,Xu, Junfei,Feng, Xiangyuan,Duan, Shengwen,Zheng, Ke,Cheng, Lifeng,Liu, Jianping,Dai, Xiaoyang,Xie, Daping,Peng, Keqing.

[16]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[17]The disruption of two salt bridges of the cold-active xylanase XynGR40 results in an increase in activity, but a decrease in thermostability. Wang, Guozeng,Wu, Jingjing,Lin, Juan,Ye, Xiuyun,Yao, Bin.

[18]A novel, alkali-tolerant thermostable xylanase from Saccharomonospora viridis: direct gene cloning, expression and enzyme characterization. Wang, Ziyuan,Jin, Yi,Wu, Huijun,Xie, Xiangming,Tian, Zhaofeng,Wu, Yuying.

[19]Utility of Thermostable Xylanases of Mycothermus thermophilus in Generating Prebiotic Xylooligosaccharides. Ma, Rui,Bai, Yingguo,Huang, Huoqing,Luo, Huiying,Yao, Bin,Ma, Rui,Fan, Yunliu,Ma, Rui,Chen, Sanfeng,Cai, Lei.

[20]Production of xylobiose from the autohydrolysis explosion liquor of corncob using Thermotoga maritima xylanase B (XynB) immobilized on nickel-chelated Eupergit C. Tan, S. S.,Li, D. Y.,Jiang, Z. Q.,Zhu, Y. P.,Shi, B.,Li, L. T..

作者其他论文 更多>>

微信小程序