文献类型： 外文期刊
第一作者： Xia, Ruixue
作者： Xia, Ruixue;Lu, Yang;Wang, Na;Zhang, Anqi;Guo, Changyou;Xu, Zhenmei;He, Yuanzheng;Sun, Mingxia;Cai, Xuehui;He, Yuanzheng
作者机构：
关键词： Heliorhodopsin; Acetate; Nanobody; Cryo-EM
期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：2.2； 五年影响因子：2.5 ）
ISSN： 0006-291X
年卷期： 2025 年 750 卷
页码：
收录情况： SCI
摘要： Heliorhodopsins (HeRs) represent a distinct class of microbial rhodopsins (MRs) with an inverted membrane topology compared to other MRs. Previous structural studies have shown that HeRs lack a proton acceptor residue, and protons are never released from the protein. In this study, we present the cryo-electron microscopy (cryo-EM) structure of HeR bound to a nanobody. The structure reveals an acetate-like molecule in the Schiff base cavity (SBC) on the intracellular side of HeR under neutral condition. Structural comparisons and analyses suggest that the acetate molecule may function as a proton acceptor for the protonated retinal Schiff base (RSB) and act as a mediator for the intramolecular signaling transduction in HeR during light stimulation. These structural insights shed new light on the mechanism and function of HeR.
分类号：
- 相关文献
作者其他论文 更多>>
-
A lineage 1 branch porcine reproductive and respiratory syndrome virus live vaccine candidate provides broad cross-protection against HP-like PRRSV in piglets
作者：Li, Chao;Li, Jinhao;Gong, Bangjun;Xu, Hu;Guo, Zhenyang;Xiang, Lirun;Zhang, Siyu;Sun, Qi;Zhao, Jing;Zhang, Menglin;Tang, Yan-Dong;Wu, Jianan;Wang, Qian;Peng, Jinmei;Zhou, Guohui;Liu, Huairan;An, Tongqing;Cai, Xuehui;Tian, Zhi-Jun;Zhang, Hongliang;Leng, Chaoliang
关键词：Lineage 1 branch; live vaccine; HP-like PRRSV; cross-protective efficacy
-
A strain of Talaromyces assiutensis provides multiple protection effects against insect pests and a fungal pathogen after endophytic settlement in soybean plants
作者：Sui, Li;Lu, Yang;Cheng, Ke;Tian, Yifan;Liu, Zhiming;Xie, Zhao;Zhang, Zhengkun;Li, Qiyun;Tian, Yifan;Li, Qiyun;Li, Qiyun
关键词：Entomopathogenic fungi; Talaromyces assiutensis; Broad-spectrum pathogenicity; Endogenous colonization; Biocontrol
-
Endophytic Beauveria bassiana promotes sunflower growth and confers resistance against sclerotinia disease
作者：Sui, Li;Lu, Yang;Yang, Huiping;Liu, Zhiming;Zou, Xiaowei;Li, Qiyun;Zhang, Zhengkun;Liu, Zhiming;Li, Qiyun;Zhang, Zhengkun;Wang, Li;Li, Qiyun
关键词：Entomopathogenic fungi; Endophytic; Promote plant growth; Disease resistance; Soil-borne disease
-
Development of blocking ELISA for detection anti-PRRSV antibodies and serological investigation of PRRSV in China
作者：Wang, Shujie;Chen, Tianfeng;Yang, Yong-Bo;Wang, Hongfeng;Sun, Mingxia;Gao, Kexin;Wei, Ziyi;Bai, Yuanzhe;Tang, Yan-Dong;Cai, Xuehui;An, Tongqing;Wang, Shujie;An, Tongqing;Cai, Xuehui
关键词：Porcine reproductive and respiratory syndrome; virus; Nucleocapsid (N) protein; Monoclonal antibodies; b-ELISA
-
Novel conjugated 5-alkenyl rhodanine tethered 1,4-benzodioxane derivatives as dual-chitinases inhibitors to hinder the growth of Asian corn borer
作者：Chen, Jinxiu;Shi, Dongmei;Jiang, Zhiyang;Zou, Renxuan;Zhang, Jingyu;Han, Qing;Wang, Na;Duan, Hongxia;Chen, Jinxiu;Shi, Dongmei;Jiang, Zhiyang;Zou, Renxuan;Zhang, Jingyu;Han, Qing;Wang, Na;Duan, Hongxia;Xu, Zhijian;Yang, Qing
关键词：O. furnacalis; Chitinase; 5-alkenylthiazolidinone; 1,4-benzodioxane; Dual-chitinases inhibitor
-
Role of the cell cycle-related gene cdk2 and its associated ceRNA network in sexual size dimorphism of Cynoglossus semilaevis
作者：Li, Xihong;Wang, Jiacheng;Mai, Jiaqi;Sun, Yuqi;Li, Wenjie;Cai, Zhenyu;Xu, Wenteng;Chen, Zhangfan;Chen, Songlin;Wang, Na;Li, Xihong;Xu, Wenteng;Chen, Zhangfan;Chen, Songlin;Wang, Na;Wang, Jiacheng;Li, Wenjie;Wang, Na
关键词：Cynoglossus semilaevis; Sexual size dimorphism; cdk2; ceRNA
-
Genome-Wide Identification and Characterization of gh/prl/sl Family in Cynoglossus semilaevis
作者：Zhang, Min;Shi, Yuhong;Wang, Zhe;Chen, Zhangfan;Li, Xihong;Xu, Wenteng;Wang, Na;Chen, Zhangfan;Li, Xihong;Xu, Wenteng;Wang, Na
关键词：
gh /prl /sl family; growth hormone receptor; prolactin receptor; Chinese tongue sole (Cynoglossus semilaevis ); pituitary
