Structural optimization, characterization, and evaluation of binding mechanism of aptamers against bovine pregnancy-associated glycoproteins and their application in establishment of a colorimetric aptasensor using Fe-based metal-organic framework as peroxidase mimic tags

文献类型： 外文期刊

第一作者： Lu, Chunxia

作者： Lu, Chunxia;Lu, Chunxia;Zhang, Zhenliang;Tang, Zonggui;Liu, Changbin;Qin, Jiaxiang;Wu, Shijia

作者机构：

关键词： Pregnancy-associated glycoproteins; Binding mechanism; Molecular dynamics; Colorimetric aptasensor; Metal-organic frameworks

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：4.9 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 11 期

页码：

收录情况： SCI

摘要： A truncated aptamer (designated A24-3) was identified that specifically binds to bovine pregnancy-associated glycoproteins (bPAG9) with a low dissociation constant (2.04 nM) through two truncation approaches. Circular dichroism spectroscopy indicated that A24-3 formed parallel G-quadruplexes, which was subsequently confirmed using nuclear magnetic resonance (NMR) spectroscopy. Furthermore, a molecular dynamics simulation was employed to investigate the recognition mechanism of A24-3 and bPAG9. Interaction analysis showed that A24-3 folded into a parallel G-quadruplex structure with three G-tetrads, primarily through numerous hydrogen bonds and hydrophobic and pi-pi interactions. Finally, a novel colorimetric aptasensor was developed for detecting bPAG9 using A24-3 and an Fe-based metal-organic framework as target recognition elements and enzyme mimics, respectively. The method demonstrated a broad detection range from 0.5 to 50 ng/mL, with a low detection limit of 0.03 ng mL-1, and exhibited a good recovery (91.0-102%) for bPAG9-spiked serum samples. Additionally, the aptasensor was successfully applied to detecting the pregnancy-specific biomarker bPAGs in serum samples.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序