Lactobacillus salivarius Ameliorates AFB1-induced hepatotoxicity via PINK1/Parkin-mediated mitophagy in Geese

文献类型： 外文期刊

第一作者： Qiu, Zhi

作者： Qiu, Zhi;Wang, Xichun;Wang, Huiying;Li, Guangquan;Liu, Yi;He, Daqian;Qiu, Zhi;Wang, Xianze;Yang, Junhua

作者机构：

关键词： Lactobacillus salivarius; Aflatoxin B1; Hepatotoxicity; Mitochondrial autophagy; PINK1 /Parkin pathway; Goose

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.2； 五年影响因子：6.3 ）

ISSN： 0147-6513

年卷期： 2024 年 280 卷

页码：

收录情况： SCI

摘要： Aflatoxin B1 (AFB1) is commonly found in feed ingredients and foods all over the world, posing a significant threat to food safety and public health in animals and humans. Lactobacillus salivarius ( L. salivarius ) was recorded to improve the intestinal health and performance of chickens. However, whether L. salivarius can alleviate AFB1induced hepatotoxicity in geese was unknown. A total of 300 Lande geese were randomly assigned to five groups: control group, AFB1 low-dose group (L), L. salivarius +AFB1 low-dose group (LL), AFB1 high dosage groups (H), L. salivarius +AFB1 high dosage groups (LH), respectively. The results showed that the concentrations of ALT, AST, and GGT significantly increased after exposure to AFB1. Similarly, severe damage of hepatic morphology was observed including the hepatic structure injury and inflammatory cell infiltration. The oxidative stress was evidenced by the elevated concentrations of MDA, and decreased activities of GSH-Px, GSH and SOD. The observation of immunofluorescence, real -time PCR, and western blotting showed that the expression of PINK1 and the value of LC3II/LC3I were increased, but that of p62 significantly decreased after AFB1 exposure. Moreover, the supplementation of L. salivarius effectively improved the geese performance, ameliorated AFB1induced oxidative stress, inhibited mitochondrial mitophagy and enhanced the liver restoration to normal level. The present study demonstrated that L. salivarius ameliorated AFB1-induced the hepatotoxicity by decreasing the oxidative stress, and regulating the expression of PINK1/Parkin-mediated mitophagy in the mitochondria of the geese liver. Furthermore, this investigation suggested that L. salivarius might serve as a novel and safe additive for preventing AFB1 contamination in poultry feed.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序