Dietary succinic acid mitigates adverse effects of starch high-starch diet in largemouth bass (Micropterus salmoides) through gut-liver axis modulation

文献类型： 外文期刊

第一作者： Liu, Yangyang

作者： Liu, Yangyang;Cao, Manxia;Zhang, Jianmin;Jiang, Ming;Cheng, Ke;Dong, Lixue;Peng, Di;Tian, Juan;Cao, Manxia;Huang, Feng;Tian, Juan;Cao, Manxia;Wang, Xuan

作者机构：

关键词： Succinic acid; High-starch diet; Glucolipid metabolism; Enterohepatic health

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.5； 五年影响因子：3.1 ）

ISSN： 0920-1742

年卷期： 2025 年 51 卷 4 期

页码：

收录情况： SCI

摘要： A 10-week feeding trial was conducted to investigate the effects of succinic acid (SUA) supplementation in high-starch diets (HSD) on growth and enterohepatic health of largemouth bass (Micropterus salmoides; initial weight 11.96 +/- 0.71 g). Six isocaloric and isolipidic diets were formulated, comprising a standard diet containing 10% corn starch and 40% fish meal, an HSD with 15% corn starch and 36% fish meal, and HSDs supplemented with 0.5%, 1.0%, 1.5%, and 2.0% SUA. Compared to standard diet, HSD exhibited adverse effects including impaired feed utilization, growth retardation, diminished antioxidant capacity, and immune response, along with metabolic dysregulation and gut microbiota disturbances. Significant linear and quadratic dose-responses were detected for multiple parameters: feed conversion ratio, hepatosomatic index, viscerosomatic index, glycogen content, amylase and protease activities, and hepatic antioxidant status. The 0.5% SUA supplementation group demonstrated enhanced intestinal morphology, upregulated expression of tight junction proteins (ZO-1 and Claudin-4) and antiapoptotic Bcl2, increased abundance of beneficial microbiota and suppressed endoplasmic reticulum stress markers (GRP78, PERK, IRE1, ATF6, eIF2 alpha, and Chop alpha). Additionally, dietary 0.5% SUA upregulated hepatic anti-inflammatory mediators (Nrf2, TGF beta 1, and IL10), glycolytic genes (PK, PFKL2, and GK), beta-oxidation-related CPT1, antiapoptotic Bag, and glucose transporter GLUT2, while downregulating proinflammatory TNF alpha, gluconeogenic enzymes (PEPCK and G6Pase), lipogenic genes (ACC1 and FASN), and proapoptotic Bad. Broken-line regression analysis identified 0.46-0.50% as the optimal SUA inclusion level based on growth parameters. This study demonstrates that appropriate SUA supplementation alleviates HSD-induced oxidative stress, enhances intestinal barrier function, modulates gut microbiota, and maintains metabolic homeostasis, thereby improving starch utilization and growth performance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序