Integrated transcriptome and co-expression analysis identified a putative miRNA64-CYP82D27 module regulating oleanane-type saponins biosynthesis in Asparagus officinalis

文献类型： 外文期刊

第一作者： Ahmad, Naveed

作者： Ahmad, Naveed;Wang, Xingjun;Zhang, Xiaoqian;Li, Guanghui;Zhao, Chuanzhi;Zhao, Shuzhen;Ahmad, Naveed;Wang, Xingjun;Zhao, Chuanzhi;Zhao, Shuzhen

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关键词： Asparagus officinalis; Transcriptome; MiRNAs; heterologous expression; Oleanane-type saponins

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：6.2； 五年影响因子：6.2 ）

ISSN： 0926-6690

年卷期： 2025 年 226 卷

页码：

收录情况： SCI

摘要： Triterpenes are a highly diverse class of plant natural products, renowned for their wide range of pharmacological activities. Asparagus officinalis is a highly valued medicinal and nutritious crop, yet the biosynthesis of its oleanane-type saponins has remained largely unexplored. This study aims to identify key functional miRNAmRNA modules involved in the biosynthetic pathway of oleanane-type saponins in A. officinalis using metabolomics and transcriptomics association analysis. LC/MS analysis of spear tips and main spears from two asparagus cultivars, Champion (JY-10) and Jinglv 3 (JY-14), revealed a significant variation in soyasaponin accumulation. Notably, the results showed that JY-14 tips contained significantly higher level of soyasaponin (8.22 mg/g) than that of JY-10 (1.54 mg/g). Subsequently, RNA-seq and small RNA-seq were performed, identifying a total of 66 miRNAs, 1794 genes, and 14 candidate miRNA-mRNA interaction modules with differential expression between the two cultivars. Furthermore, the weighted gene expression correlation network analysis (WGCNA) identified two co-expression modules, MEblue and MEturquoise, that showed strong positive correlation with soyasaponins (oleanane-type). Within the MEblue module, a putative miRNA-mRNA module (miRNA64-CYP82D27) emerged as a significant module likely correlated with soyasaponins. Transient expression experiment showed that miRNA64-CYP82D27 complex exhibits catalytic activity and converting oleanolic acid into soyasaponins. These findings imply that miRNA64-CYP82D27 module plays a key role in oleanane-type saponins biosynthesis in A. officinalis, offering valuable insight for future research on triterpenoid biosynthesis and asparagus breeding improvement.

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