Sphaerisporangium perillae sp. nov., isolated from the root of Perilla frutescens (Linn.) Britt

文献类型： 外文期刊

第一作者： Wang, Teng

作者： Wang, Teng;Gao, Congting;Chen, Hongmin;Yang, Jingquan;Du, Rui;Zuo, Gengxin;Zhao, Junwei;Xiang, Wensheng;Liu, Chongxi;Xiang, Wensheng

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关键词： Sphaerisporangium perillae sp; nov; Perilla frutescens (Linn; ) Britt; protease

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 1466-5026

年卷期： 2023 年 73 卷 4 期

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收录情况： SCI

摘要： A novel protease-producing actinomycete, designated strain NEAU- ZS1T, was isolated from the root of Perilla frutescens (Linn.) Britt collected from Jiamusi, Heilongjiang, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU- ZS1T belonged to the genus Sphaerisporangium and was most closely related to 'Sphaerisporangium corydalis' NEAU-YHS15T (99.2%) and Sphaerisporangium cinnabarinum JCM 3291T (99.0%). Phylogenetic tree analysis revealed that strain NEAU- ZS1T formed a monophyletic clade with 'S. corydalis' NEAU-YHS15T. The genome size was 9.3 Mbp with a DNA G+C content of 70.3 mol%. Digital DNA-DNA hybridization, average nucleotide identity and average amino acid identity values between the genome sequence of strain NEAU-ZS1T and those of 'S. corydalis' NEAU-YHS15T (28.6, 83.9 and 79.1 %) and S. cinnabarinum JCM 3291T (18.5, 70.6 and 50.2 %) were below the recommended thresholds for species delineation. The strain formed spherical spore vesicles produced on the aerial hyphae. The cell wall contained meso- diaminopimelic acid and the whole -cell sugars were glucose and madu-rose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phos-phatidylinositol, an unidentified phospholipid and an unidentified glycolipid. The menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The major fatty acids were iso-C16:0, C16: 1 omega 5c, 10- methy C17: 0 and C17: 1 omega 7c. On the basis of the results of a polyphasic taxonomic study, it is concluded that strain NEAU- ZS1T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium perillae sp. nov. is proposed. The type strain is NEAU- ZS1T (=CCTCC AA 2021019T= JCM 35655T).

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