Molecular and Biological Characterization of Novel Mitovirus Infecting Phytopathogenic Fungus Ustilaginoidea virens

文献类型： 外文期刊

第一作者： He, Zhenrui

作者： He, Zhenrui;Zhao, Wenhua;Yang, Mei;Zhu, Yiming;Hou, Erxun;Cheng, Baoping;Yang, Yingqing

作者机构：

关键词： rice false smut; Ustilaginoidea virens; mitovirus; autophagy; biocontrol

期刊名称：RICE SCIENCE （ 影响因子：6.1； 五年影响因子：5.6 ）

ISSN： 1672-6308

年卷期： 2025 年 32 卷 2 期

页码：

收录情况： SCI

摘要： Rice false smut (RFS), caused by the ascomycete fungus Ustilaginoidea virens (Cooke) Takahashi, is a notable panicle disease of rice. Mycoviruses represent a category of viruses capable of infecting various fungal groups, garnering interest for their potential application in the biological control of fungal diseases. We identified a novel positive-sense single-stranded RNA virus, named Ustilaginoidea virens mitovirus 1 (UvMV1), from an abnormal U. virens strain Uv488. The complete genome sequence of UvMV1 is 2 474 nt in length and contains a large open reading frame encoding RNA-dependent polymerase. UvMV1 is classified as a new member of the genus Unuamitovirus in the family Mitoviridae based on phylogenetic analysis. It is effectively transmitted vertically through conidia of strain Uv488 horizontally through hyphal fusion between vegetatively compatible individuals with an 80% transmission efficiency. We further demonstrated that UvMV1 significantly influenced conidiation, colony morphology, growth rate, secondary metabolite production, and the response to environmental stress in U. Furthermore, qRT-PCR results aimed at detecting the expression levels of autophagy-related suggested that UvMV1 infection had the potential to induce autophagy in U. virens. Activation or inhibition of autophagy through chemical experiments demonstrated that UvMV1 enhances viral titers by inducing autophagy in U. virens, while inhibition of autophagy results in decreased UvMV1 titers. Transcriptome analyses consistently demonstrated that UvMV1 regulated the expression of genes associated secondary metabolism, mycelial growth, virulence, and the mitogen-activated protein kinase signaling pathway, thereby influencing both the antiviral response and the virulence of U. virens. The presented collectively identified a novel mycovirus responsible for inducing growth abnormalities virens, and elucidated the regulatory mechanisms during mycovirus-host fungus interactions, particularly the autophagy pathway. These findings offer new perspectives and potential control strategies for managing RFS in agricultural settings.

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