Young Leaf White Stripe encodes a P-type PPR protein required for chloroplast development

文献类型： 外文期刊

第一作者： Lan, Jie

作者： Lan, Jie;Zhou, Chunlei;Liu, Xi;Miao, Rong;Ma, Tengfei;Chen, Yaping;Mou, Changling;Jing, Ruonan;Nguyen, Thanhliem;Liu, Shijia;Jiang, Ling;Wan, Jianmin;Lin, Qibing;Feng, Miao;Ren, Yulong;Cheng, Zhijun;Zhang, Xin;Wan, Jianmin

作者机构：

关键词： chloroplast development; PPR protein; ribosome biogenesis; RNA splicing

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Pentatricopeptide repeat (PPR) proteins function in post-transcriptional regulation of organellar gene expression. Although several PPR proteins are known to function in chloroplast development in rice (Oryza sativa), the detailed molecular functions of many PPR proteins remain unclear. Here, we characterized a rice young leaf white stripe (ylws) mutant, which has defective chloroplast development during early seedling growth. Map-based cloning revealed that YLWS encodes a novel P-type chloroplast-targeted PPR protein with 11 PPR motifs. Further expression analyses showed that many nuclear- and plastid-encoded genes in the ylws mutant were significantly changed at the RNA and protein levels. The ylws mutant was impaired in chloroplast ribosome biogenesis and chloroplast development under low-temperature conditions. The ylws mutation causes defects in the splicing of atpF, ndhA, rpl2, and rps12, and editing of ndhA, ndhB, and rps14 transcripts. YLWS directly binds to specific sites in the atpF, ndhA, and rpl2 pre-mRNAs. Our results suggest that YLWS participates in chloroplast RNA group II intron splicing and plays an important role in chloroplast development during early leaf development.

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