Silencing CaPIP5K4-1 leads to decreased male fertility in Capsicum annuum L.

文献类型： 外文期刊

第一作者： Kong, Weifu

作者： Kong, Weifu;Wang, Yuhang;Zhang, Tao;Huang, Mianzhu;Kang, Jingtao;Wang, Lina;Wei, Bingqiang;Duan, Panpan;Chang, Yajun

作者机构：

关键词： Male fertility; Pepper; Phosphatidylinositol 4-phosphate 5-kinase; Virus; Induced gene silencing

期刊名称：PLANTA （ 影响因子：3.8； 五年影响因子：4.4 ）

ISSN： 0032-0935

年卷期： 2025 年 261 卷 1 期

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收录情况： SCI

摘要： Main conclusionPhosphatidylinositol 4-phosphate 5-kinase gene CaPIP5K4-1 is highly expressed in the pepper anthers. Virus-induced gene silencing of CaPIP5K4-1 leads to reduced male fertility in pepper.AbstractThe phosphatidylinositol 4-phosphate 5-kinase (PIP5K) is a pivotal enzyme in the phosphatidylinositol signaling pathway, and its crucial involvement in both plant development and stress response has been established. Here, we found that the expression of CaPIP5K4-1 in pepper was significantly higher in the fertile flower buds compared to sterile flower buds. Furthermore, its expression was validated in anthers and pollens by qRT-PCR and RNA-ISH assays, respectively. Its GFP fusion protein was mainly located on the plasma membrane. Silencing CaPIP5K4-1 in fertile pepper accessions resulted in wrinkled pollen grain cell walls, decreased pollen germination efficiency, and inhibited pollen tube growth. The transcription levels of multiple genes in the phosphatidylinositol signaling pathway were also assessed. Five phospholipase C (PLC) genes were downregulated in silenced plants. On the contrary, inositol phosphatase SAC and phosphatase and tensin homolog (PTEN) were upregulated. This study reported the role of CaPIP5K4-1 in pepper male fertility and provided insights into the regulatory mechanisms of PI signaling in pepper.

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