Sucrose induces BoVDAC2 expression to preserve mitochondrial function and mitigate programmed cell death in broccoli

文献类型： 外文期刊

第一作者： Luo, Min

作者： Luo, Min;Chen, Jiahui;Guo, Xintong;Qian, Yuan;Jiao, Wenhui;Chen, Yi;Wei, Yingying;Shao, Xingfeng;Xu, Feng;Cao, Shifeng;Chen, Hangjun

作者机构：

关键词： Broccoli; PCD; VDAC; Mitochondria; BY-2 cells; Oxidative stress

期刊名称：POSTHARVEST BIOLOGY AND TECHNOLOGY （ 影响因子：6.8； 五年影响因子：7.5 ）

ISSN： 0925-5214

年卷期： 2025 年 222 卷

页码：

收录情况： SCI

摘要： The alleviation of the impact of sucrose treatment on programmed cell death (PCD) in postharvest broccoli has been reported in previous studies; however, the fundamental mechanisms are still not fully understood. This study found that exogenous sucrose treatment maintained the mitochondrial structure in broccoli buds, enhanced mitochondrial membrane potential (Delta Psi m), elevated activity of antioxidant enzymes and decreased levels of reactive oxygen species (ROS). These changes ultimately inhibited the release of Cytochrome c/a (Cytc/ a) and delayed PCD in broccoli buds. Furthermore, the expression of BoVDAC2 progressively decreased with prolonged storage. To further explore the relationship between BoVDAC2 and PCD, BoVDAC2 was overexpressed in BY-2 cells in this study. Compared to wild-type cells, BoVDAC2-overexpressing cells showed reduced mitochondrial ultrastructure damage, increased cell vitality, enhanced antioxidant enzyme activity, and decreased ROS production. Additionally, BoVDAC2 overexpression elevated energy metabolism, maintained mitochondrial membrane integrity, inhibited Cytc/a release, suppressed caspase-like activity, and improved the expression of genes related to PCD in BY-2 cells. Overall, the findings suggest that sucrose induces BoVDAC2 expression, which helps to maintain mitochondrial structure and function in postharvest broccoli buds, thereby mitigating PCD.

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